Overview

  • Product nameAnti-MMP7 antibody
    See all MMP7 primary antibodies
  • Description
    Rabbit polyclonal to MMP7
  • SpecificityAb38997 recognizes a site midway between the aminoterminal end and the zinc site (active site) of MMP7. It does not cross react with the other MMP family members.
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Synthetic peptide corresponding to a site midway between the aminoterminal end and the zinc site of human MMP7.

    (Peptide available as ab41057.)

  • Positive control
    • WB: Human MMP7 and cell media from pig kidney, epithelial morphology (treated with IL1 alpha).

Properties

Applications

Our Abpromise guarantee covers the use of ab38997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 29 kDa.Can be blocked with MMP7 peptide (ab41057). A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Note: MMP7 does not appear to be produced by most normal quiescent cells, but treatment of many cell types with the phorbol ester TPA stimulates its production. Due to the low protein levels produced (pg/mL) concentration of cell culture media is often required to visualize the bands.

Target

  • FunctionDegrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
  • Sequence similaritiesBelongs to the peptidase M10A family.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Matrilysin antibody
    • Matrilysin uterine antibody
    • Matrin antibody
    • Matrix Metalloproteinase 7 antibody
    • Matrix metalloproteinase-7 antibody
    • MMP 7 antibody
    • MMP-7 antibody
    • MMP7 antibody
    • MMP7_HUMAN antibody
    • MPSL1 antibody
    • PUMP 1 antibody
    • Pump 1 protease antibody
    • Pump-1 protease antibody
    • PUMP1 antibody
    • Uterine Matrilysin antibody
    • Uterine metalloproteinase antibody
    see all

Anti-MMP7 antibody images

  • All lanes : Anti-MMP7 antibody (ab38997) at 1/1000 dilution

    Lane 1 : Human MMP-7
    Lane 2 : Cell media from Pig kidney (treated with IL1 alpha)


    Predicted band size : 29 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa (possible dimer).
  • Ab38997 staining human normal placenta tissue. Staining is localised to extracellular space.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-MMP7 antibody (ab38997)

ab38997 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"