MMP7 antibody - Middle of active MMP-7 (ab38997)

Product Name 

MMP7 antibody - Middle of active MMP-7

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MMP7 - Middle of active MMP-7

Immunogen 

Synthetic peptide corresponding to a site midway between the aminoterminal end and the zinc site of human MMP7.

(Peptide available as ab41057.)

Reacts with 
(species key)

Hu, Pig

Specificity 

Ab38997 recognizes a site midway between the aminoterminal end and the zinc site (active site) of MMP7. It does not cross react with the other MMP family members.

We have a range of domain specific antibodies for this target. For a full list please see all MMP7 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 29 kDa. When used against the reduced protein, ab38997 identifies bands at about 28 kDa and 18 kDa (the pro form and active forms). Note: MMP7 does not appear to be produced by most normal quiescent cells, but treatment of many cell types with the phorbol ester TPA stimulates its production. Because of the low protein levels produced (pg/mL) concentration of cell culture media is often required to visualize the bands by Western Blotting. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: Human MMP7 and cell media from pig kidney, epithelial morphology (treated with IL1 alpha).

Cellular localization 

Extracellular space

Research areas 

Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

Relevance 

Matrix Metalloproteinases (MMPs) belong to a family of proteases that are essential for the breakdown of extracellular matrix (ECM). They are thus important in apoptosis, tumor cell growth, invasion, and metastasis, as well as angiogenesis and wound healing. Most MMPs contain common domain structures that include a signal sequence, a propeptide, a catalytic domain, and a hemopexin like (Hpx) domain. MMP7, also known as Matrilysin, is responsible for hydrolyzing proteoglycans and ECM glycoproteins. It is expressed in normal cells, specifically glandular epithelial cells. MMP7, is thought to play an important role in the early steps of tumor progression. Expression of MMP2, MMP7 and MMP11 is greater in pancreatic carcinoma than in normal pancreas.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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