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Western blot - MMP7 antibody (ab38996)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-MMP7 antibody - Propeptide domain(ab38996)
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Product Name
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MMP7 antibody - Propeptide domain
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See all MMP7 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MMP7 - Propeptide domain
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Immunogen
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Synthetic peptide based on the propeptide region of human MMP7.
(Peptide available as ab41061.)
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Reacts with
(species key)
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Hu, Pig
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Specificity
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Ab38996 is directed to the propeptide region of MMP7, which is removed during activation of the enzyme. It binds to both the native and reduced proteins. The 18 kDa MMP7 active form is not detected, thus ab38996 can be used to discriminate between latent and active MMP7. The antibody does not cross react with the other MMP family members.
We have a range of domain specific antibodies for this target. For a full list please see all MMP7 antibodies
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at an assay dependent dilution.
WB: Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 29 kDa. When used against the reduced protein, ab38996 identifies a band at about 28 kDa. Note: MMP7 does not appear to be produced by most normal quiescent cells, but treatment of many cell types with the phorbol ester TPA stimulates its production. Because of the low protein levels produced (pg/mL) concentration of cell culture media is often required to visualize the bands by Western blotting. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: Human MMP7 and cell media from pig kidney epithelia morphology (treated with IL1 alpha).
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Cellular localization
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Extracellular space
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Research areas
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Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Relevance
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Matrix Metalloproteinases (MMPs) belong to a family of proteases that are essential for the breakdown of extracellular matrix (ECM). They are thus important in apoptosis, tumor cell growth, invasion, and metastasis, as well as angiogenesis and wound healing. Most MMPs contain common domain structures that include a signal sequence, a propeptide, a catalytic domain, and a hemopexin like (Hpx) domain. MMP7, also known as Matrilysin, is responsible for hydrolyzing proteoglycans and ECM glycoproteins. It is expressed in normal cells, specifically glandular epithelial cells. MMP7, is thought to play an important role in the early steps of tumor progression. Expression of MMP2, MMP7 and MMP11 is greater in pancreatic carcinoma than in normal pancreas.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MMP7 antibody - Propeptide domain is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MMP7 antibody - Propeptide domain images, references, products related to ab38996 and other tools.
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MMP7 antibody - Propeptide domain images:
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 Western blot - MMP7 antibody (ab38996)
All lanes : MMP7 antibody - Propeptide domain (ab38996) at 1/1000 dilution
Lane 1 : Human MMP7
Lane 2 : Cell media from pig kidney, epithelial morphology (treated with IL1 alpha).
Predicted band size : 29 kDa
Observed band size : 23,29 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-MMP7 antibody - Propeptide domain(ab38996)
Ab38996 staining human normal placenta tissue. Staining is localised to extracellular space.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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References for MMP7 antibody - Propeptide domain (ab38996)
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This product has been used in:
Dixon JA et al. Mesenchymal cell transplantation and myocardial remodeling after myocardial infarction. Circulation 120:S220-9 (2009). PubMed: 19752372
If you publish research using ab38996 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MMP7
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MMP7 antibody - Propeptide domain - more information
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MMP7 antibody - Propeptide domain - related products:
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MMP7 antibody - Propeptide domain - other tools:
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Contact Abcam with a Technical Enquiry about MMP7 antibody - Propeptide domain |
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