Overview

  • Product name
  • Description
    Rabbit polyclonal to MMP8
  • Specificity
    MMP8 antibody detects endogenous levels of total MMP8 protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, Flow Cyt, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from human MMP8

  • Positive control
    • Human breast carcinoma tissue; extracts from NIH/3T3 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab53017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 21133852ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration. PubMed: 21133852
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • Function
    Can degrade fibrillar type I, II, and III collagens.
  • Tissue specificity
    Neutrophils.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localization
    Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLG 1 antibody
    • CLG1 antibody
    • Collagenase 1 antibody
    • Collagenase 1 neutrophil antibody
    • HNC antibody
    • Matrix metallopeptidase 8 (neutrophil collagenase) antibody
    • Matrix metalloprotease 8 antibody
    • Matrix metalloproteinase-8 antibody
    • MMP 8 antibody
    • MMP-8 antibody
    • Mmp8 antibody
    • MMP8_HUMAN antibody
    • Neutrophil collagenase antibody
    • PMNL CL antibody
    • PMNL collagenase antibody
    • PMNL-CL antibody
    • PMNLCL antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MMP8 antibody at 1/50 dilution. Left hand image; untreated tissue, Right hand image; tissue treated with the immunising peptide.
  • All lanes : Anti-MMP8 antibody (ab53017) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells with no immunizing peptide
    Lane 2 : Extracts from NIH/3T3 cells with immunizing peptide


    Predicted band size : 53 kDa
    Observed band size : 53 kDa
  • ICC/IF image of ab63017 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53017, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Oberkofler CE  et al. Systemic protection through remote ischemic preconditioning is spread by platelet-dependent signaling in mice. Hepatology 60:1409-17 (2014). Read more (PubMed: 24700614) »
  • Xiao Q  et al. Functional role of matrix metalloproteinase-8 in stem/progenitor cell migration and their recruitment into atherosclerotic lesions. Circ Res 112:35-47 (2013). Read more (PubMed: 23071158) »

See all 4 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer pH 6.0
Sample
Mouse Tissue sections (liver)
Specification
liver
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 04 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Non-Denaturing (Native)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (human gut)
Specification
human gut
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Feb 14 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (rat vascular smooth muscle cells)
Loading amount
100000 cells
Specification
rat vascular smooth muscle cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Feb 05 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.


I am sorry these products did not perform as stated on the datasheet and for the inconveni...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (human gut)
Loading amount
50 µg
Specification
human gut
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jul 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lymphoma)
Specification
lymphoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer (pH 6.0)
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 12 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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