The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
Use at an assay dependent concentration.
Use at an assay dependent concentration. PubMed: 21133852ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration. PubMed: 21133852
Use a concentration of 1 - 5 µg/ml.
Can degrade fibrillar type I, II, and III collagens.
Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.
Immunohistochemical analysis of paraffin-embedded
human breast carcinoma tissue using MMP8 antibody at 1/50 dilution. Left hand image; untreated tissue, Right hand image; tissue treated with the immunising peptide.
Western blot - MMP8 antibody (ab53017)
All lanes : Anti-MMP8 antibody (ab53017) at 1/500 dilution
Lane 1 : Extracts from NIH/3T3 cells with no immunizing peptide Lane 2 : Extracts from NIH/3T3 cells with immunizing peptide
Predicted band size : 53 kDa Observed band size : 53 kDa
ICC/IF image of ab63017 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53017, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Oberkofler CE et al. Systemic protection through remote ischemic preconditioning is spread by platelet-dependent signaling in mice. Hepatology60:1409-17 (2014).
Read more (PubMed: 24700614) »
Xiao Q et al. Functional role of matrix metalloproteinase-8 in stem/progenitor cell migration and their recruitment into atherosclerotic lesions. Circ Res112:35-47 (2013).
Read more (PubMed: 23071158) »