Overview

  • Product name
  • Description
    Rabbit polyclonal to MMP8
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human MMP8.

    (Peptide available as ab94385.)

  • Positive control
    • This antibody gave a positive signal in the following human lysates: HeLa whole cell; bone marrow tissue; skin tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab72897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Can degrade fibrillar type I, II, and III collagens.
    • Tissue specificity
      Neutrophils.
    • Sequence similarities
      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • Domain
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Cellular localization
      Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.
    • Information by UniProt
    • Database links
    • Alternative names
      • CLG 1 antibody
      • CLG1 antibody
      • Collagenase 1 antibody
      • Collagenase 1 neutrophil antibody
      • HNC antibody
      • Matrix metallopeptidase 8 (neutrophil collagenase) antibody
      • Matrix metalloprotease 8 antibody
      • Matrix metalloproteinase-8 antibody
      • MMP 8 antibody
      • MMP-8 antibody
      • Mmp8 antibody
      • MMP8_HUMAN antibody
      • Neutrophil collagenase antibody
      • PMNL CL antibody
      • PMNL collagenase antibody
      • PMNL-CL antibody
      • PMNLCL antibody
      see all

    Images

    • All lanes : Anti-MMP8 antibody (ab72897) at 1 µg/ml

      Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : Human skin tissue lysate - total protein (ab30166)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 53 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)


      Exposure time : 2 minutes

    References

    ab72897 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab72897.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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