Overview

  • Product nameAnti-MMP8 antibody
    See all MMP8 primary antibodies
  • Description
    Rabbit polyclonal to MMP8
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human MMP8.

    (Peptide available as ab99162.)

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates:lung; testis; ovary; and in the following whole cell lysates: MOLT4; Jurkat; Raji.

Properties

Applications

Our Abpromise guarantee covers the use of ab89834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

Target

  • FunctionCan degrade fibrillar type I, II, and III collagens.
  • Tissue specificityNeutrophils.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationCytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLG 1 antibody
    • CLG1 antibody
    • Collagenase 1 antibody
    • Collagenase 1 neutrophil antibody
    • HNC antibody
    • Matrix metallopeptidase 8 (neutrophil collagenase) antibody
    • Matrix metalloprotease 8 antibody
    • Matrix metalloproteinase-8 antibody
    • MMP 8 antibody
    • MMP-8 antibody
    • Mmp8 antibody
    • MMP8_HUMAN antibody
    • Neutrophil collagenase antibody
    • PMNL CL antibody
    • PMNL collagenase antibody
    • PMNL-CL antibody
    • PMNLCL antibody
    see all

Anti-MMP8 antibody images

  • All lanes : Anti-MMP8 antibody (ab89834) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : Human ovary tissue lysate - total protein (ab30222)
    Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 6 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References for Anti-MMP8 antibody (ab89834)

ab89834 has not yet been referenced specifically in any publications.

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I have not yet heard back from the lab regarding ab81286, but hopefully later today.

However,I checked the homology between human and mousefor the sequence range101-284:it is83%, with poor alignment in ...

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