Overview

  • Product nameAnti-MMP8 antibody [EP1252Y]
    See all MMP8 primary antibodies
  • Description
    Rabbit monoclonal [EP1252Y] to MMP8
  • SpecificityWB positive for Human recombinant MMP8 but negative results for the following cell lines and tissue: HeLa, A431,K562 and bone marrow.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in human MMP8.

  • Positive control
    • Rat lung lysate. Human placenta tissue. Human urinary bladder carcinoma tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Applications

Our Abpromise guarantee covers the use of ab81286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/5000. Predicted molecular weight: 53 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

Target

  • FunctionCan degrade fibrillar type I, II, and III collagens.
  • Tissue specificityNeutrophils.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationCytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLG 1 antibody
    • CLG1 antibody
    • Collagenase 1 antibody
    • Collagenase 1 neutrophil antibody
    • HNC antibody
    • Matrix metallopeptidase 8 (neutrophil collagenase) antibody
    • Matrix metalloprotease 8 antibody
    • Matrix metalloproteinase-8 antibody
    • MMP 8 antibody
    • MMP-8 antibody
    • Mmp8 antibody
    • MMP8_HUMAN antibody
    • Neutrophil collagenase antibody
    • PMNL CL antibody
    • PMNL collagenase antibody
    • PMNL-CL antibody
    • PMNLCL antibody
    see all

Anti-MMP8 antibody [EP1252Y] images

  • Immunofluorescence staining of HL-60 cells with purified ab81286 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

  • Anti-MMP8 antibody [EP1252Y] (ab81286) at 1/2000 dilution + Rat lung lysate at 10 µg

    Secondary
    HRP conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size : 53 kDa
    Observed band size : 72 kDa (why is the actual band size different from the predicted?)
  • ab81286, at 1/100 dilution, staining MMP8 in human placenta by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
  • ab81286, at 1/100 dilution, staining MMP8 in human urinary bladder carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

References for Anti-MMP8 antibody [EP1252Y] (ab81286)

This product has been referenced in:
  • Luo K  et al. Association between postmenopausal osteoporosis and experimental periodontitis. Biomed Res Int 2014:316134 (2014). IHC-P ; Rat . Read more (PubMed: 24683547) »
  • Villalta PC  et al. Role of MMP2 and MMP9 in TRPV4-induced lung injury. Am J Physiol Lung Cell Mol Physiol 307:L652-9 (2014). Read more (PubMed: 25150065) »

See all 8 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rhesus monkey Tissue sections (Colon)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: High pH followed by low pH buffer
Permeabilization Yes - 0.1% Triton-X-100
Specification Colon
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Z-fix
Username

Abcam user community

Verified customer

Submitted Sep 30 2015

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 27 2015

Thank you for your patience.

I have heard back regarding ab81286 and have goodnews:

Yes, the immunogen for ab81286 is located between residues 101-284. The specific immunogen region aligns 100% with mouse.

I think this is...

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Thank you for contacting us.

I have not yet heard back from the lab regarding ab81286, but hopefully later today.

However,I checked the homology between human and mousefor the sequence range101-284:it is83%, with poor alignment in ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293 cells transfected with human MMP8)
Loading amount 30 µg
Specification HEK293 cells transfected with human MMP8
Treatment cell lysate (TRC), conditioned media (TRM)
Gel Running Conditions Non-reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Mar 24 2010

Thank you for contacting us.

I have had a chance to review the images which you sent to me. It looks to me that each product appears to be near the correct size however non specific bands and background makes determining the whether each i...

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