Overview

  • Product name
    Anti-MMP8 antibody [EP1252Y]
    See all MMP8 primary antibodies
  • Description
    Rabbit monoclonal [EP1252Y] to MMP8
  • Specificity
    WB positive for Human recombinant MMP8 but negative results for the following cell lines and tissue: HeLa, A431,K562 and bone marrow.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in human MMP8.

  • Positive control
    • Rat lung lysate. Human placenta tissue. Human urinary bladder carcinoma tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Applications

Our Abpromise guarantee covers the use of ab81286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/5000. Predicted molecular weight: 53 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

Target

  • Function
    Can degrade fibrillar type I, II, and III collagens.
  • Tissue specificity
    Neutrophils.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localization
    Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLG 1 antibody
    • CLG1 antibody
    • Collagenase 1 antibody
    • Collagenase 1 neutrophil antibody
    • HNC antibody
    • Matrix metallopeptidase 8 (neutrophil collagenase) antibody
    • Matrix metalloprotease 8 antibody
    • Matrix metalloproteinase-8 antibody
    • MMP 8 antibody
    • MMP-8 antibody
    • Mmp8 antibody
    • MMP8_HUMAN antibody
    • Neutrophil collagenase antibody
    • PMNL CL antibody
    • PMNL collagenase antibody
    • PMNL-CL antibody
    • PMNLCL antibody
    see all

Images

  • Immunofluorescence staining of HL-60 cells with purified ab81286 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

  • Anti-MMP8 antibody [EP1252Y] (ab81286) at 1/2000 dilution + Rat lung lysate at 10 µg

    Secondary
    HRP conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size : 53 kDa
    Observed band size : 72 kDa (why is the actual band size different from the predicted?)
  • ab81286, at 1/100 dilution, staining MMP8 in human placenta by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
  • ab81286, at 1/100 dilution, staining MMP8 in human urinary bladder carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

References

This product has been referenced in:
  • McKay TB  et al. Acute hypoxia influences collagen and matrix metalloproteinase expression by human keratoconus cells in vitro. PLoS One 12:e0176017 (2017). WB ; Human . Read more (PubMed: 28426715) »
  • Sun J  et al. Effect of simvastatin on MMPs and TIMPs in cigarette smoke-induced rat COPD model. Int J Chron Obstruct Pulmon Dis 12:717-724 (2017). IHC-P ; Rat . Read more (PubMed: 28260878) »

See all 13 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: High pH followed by low pH buffer
Permeabilization
Yes - 0.1% Triton-X-100
Specification
Colon
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Z-fix
Username

Abcam user community

Verified customer

Submitted Sep 30 2015

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (MCF7)
Specification
MCF7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 27 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cells transfected with human MMP8)
Loading amount
30 µg
Specification
HEK293 cells transfected with human MMP8
Treatment
cell lysate (TRC), conditioned media (TRM)
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Mar 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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