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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP8 antibody - Hinge region
See all MMP8 products (12) ...
Description
Rabbit polyclonal to MMP8 - Hinge region
Specificity
Ab38994 recognises the hinge region of MMP8. It does not cross react with the other MMP family members.
Tested applications
WB, Sandwich ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the hinge region of the Human MMP8.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Sandwich ELISA - MMP8 antibody - Hinge region (ab38994)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab38994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
- 1 Image
Sandwich ELISA
Application notes
WB: Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 53 kDa. When used against the reduced protein, ab38994 identifies bands at 64 kDa, 58 kDa and a series of further cleaved active forms. Note: MMP8 levels in quiescent cells and tissues are minimal, and mitogen stimulation or protein concentration is often needed to visualize the bands. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Can degrade fibrillar type I, II, and III collagens.
Tissue specificity
Neutrophils.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.
Target information above from: UniProt accessionP22894
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CLG 1 antibody
- CLG 1 antibody
- CLG1 antibody
see all
Database links
- Entrez Gene: 4317 Human
- Omim: 120355 Human
- SwissProt: P22894 Human
- Unigene: 161839 Human
Anti-MMP8 antibody - Hinge region images:
Sandwich ELISA - MMP8 antibody - Hinge region (ab38994)
Standard curve for MMP8; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [MM0023-7A11] to MMP8 (ab56303) at 5µg/ml and Detector Antibody Rabbit polyclonal to MMP8 - Hinge region (ab38994) at 0.5µg/ml.
References for Anti-MMP8 antibody - Hinge region (ab38994)
ab38994 has not yet been referenced specifically in any publications.
Publishing research using ab38994? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"