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Immunocytochemistry/ Immunofluorescence-MMP8 antibody - Propeptide domain(ab38988)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-MMP8 antibody - Propeptide domain(ab38988)
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Product Name
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MMP8 antibody - Propeptide domain
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See all MMP8 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MMP8 - Propeptide domain
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Immunogen
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Synthetic peptide based on the propeptide region of human MMP8.
(Peptide available as ab41066.)
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Reacts with
(species key)
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Hu
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Specificity
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Ab38988 recognises the propeptide region of MMP8. It recognizes only latent MMP8, and not any of the active forms, thus this antibody can be used to distinguish the zymogen from activated enzyme. The antibody also binds to both the reduced and non reduced protein MMP8. It does not cross react with the other MMP family members.
We have a range of domain specific antibodies for this target. For a full list please see all MMP8 antibodies
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Tested applications
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1-5 µg/ml.
WB: 1/1000 - 1/5000. Predicted molecular weight: 53 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Extracellular space and stored in intracellular granules.
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Research areas
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Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Relevance
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Matrix Metalloproteinase 8 (MMP8) is also known as neutrophil collagenase and collagenase 2. MMP8 degrades fibrillar collagens types I, II, III, aggrecan, serpins and alpha 2 macroglobulin. All collagenases cleave fibrillar collagens at one specific site resulting in generation of N terminal three quarter and C terminal one quarter fragments, which then denature to gelatin at body temperature. The substrate specificity of collagenases is variable: MMP1 degrades type III collagen more efficiently than type I or type II collagen, whereas MMP8 is more potent in degrading type I collagen than type III or type II collagen. MMP13, in turn degrades type II collagen 6 fold more efficiently than type I and type II collagens and displays almost 50 fold stronger gelatinolytic activity than MMP1 and MMP8. MMP8 is very similar to MMP1, sharing 57 % amino acid identity. Most cell types do not produce MMP8. Until recently, it was thought that MMP8 was produced exclusively by neutrophils, but it has also been detected in other cell types including arthritic chondrocytes and gingival fibroblasts. The human MMP8 gene has the chromosomal location of 11q22.2-22.3. MMP8 is heavily glycosylated, and the zymogen has a mass of 85 Kd. The zymogen is quickly activated to the 64 Kd form, and this breaks down to a cascade of active forms.
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MMP8 antibody - Propeptide domain (ab38988) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MMP8 antibody - Propeptide domain is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MMP8 antibody - Propeptide domain images, references, products related to ab38988 and other tools.
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MMP8 antibody - Propeptide domain images:
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 Immunocytochemistry/ Immunofluorescence-MMP8 antibody - Propeptide domain(ab38988)
ICC/IF image of ab38988 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38988, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-MMP8 antibody - Propeptide domain(ab38988)
Ab38988 staining human normal spleen. Staining is localized to extracellular space and stored in intracellular granules.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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Search PubMed (MEDLINE) for references to MMP8
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MMP8 antibody - Propeptide domain - more information
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MMP8 antibody - Propeptide domain - other tools:
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