Overview

  • Product nameMMP8 Human ELISA KitSee all MMP8 kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 6 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.44 84% - 103%
    Serum 94.75 83% - 103%
    Plasma 97.13 85% - 104%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s MMP8 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP8 in serum, plasma, and cell culture supernatants. (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended).

    This assay employs an antibody specific for Human MMP8 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCan degrade fibrillar type I, II, and III collagens.
  • Tissue specificityNeutrophils.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationCytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.
  • Information by UniProt
  • Alternative names
    • BB138268
    • CLG 1
    • CLG1
    • Collagenase 1
    • Collagenase 1 neutrophil
    • HNC
    • Matrix metallopeptidase 8
    • Matrix metalloproteinase 8 (neutrophil collagenase)
    • Matrix metalloproteinase-8
    • MMP 8
    • MMP-8
    • Mmp8
    • MMP8_HUMAN
    • Neutrophil collagenase
    • PMNL CL
    • PMNL collagenase
    • PMNL-CL
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

MMP8 Human ELISA Kit images

  • Representative Standard Curve using ab100609.

Protocols

References for MMP8 Human ELISA Kit (ab100609)

ab100609 has not yet been referenced specifically in any publications.

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ab100609 : Cross reactivity with mouse is unknown. If it helps, the capture antibody is monoclonal and the detection antibody is polyclonal.

ab100779 : Untes...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"