• Product nameAnti-MMP9 antibody [2C3]
    See all MMP9 primary antibodies
  • Description
    Mouse monoclonal [2C3] to MMP9
  • Specificityab53296 targets MMP9.
  • Tested applicationsSuitable for: WB, Inhibition Assay, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide:


    conjugated to Ovalbumin, corresponding to C terminal amino acids 603-614 of Human MMP9



Our Abpromise guarantee covers the use of ab53296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 78 kDa.
Inhibition Assay Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.


  • FunctionMay play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • Tissue specificityProduced by normal alveolar macrophages and granulocytes.
  • Involvement in diseaseIntervertebral disc disease
    Metaphyseal anadysplasia 2
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • Cellular localizationSecreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 82 kDa matrix metalloproteinase-9 antibody
    • 92 kDa gelatinase antibody
    • 92 kDa type IV collagenase antibody
    • CLG 4B antibody
    • CLG4B antibody
    • Collagenase Type 4 beta antibody
    • Collagenase type IV 92 KD antibody
    • EC antibody
    • Gelatinase 92 KD antibody
    • Gelatinase B antibody
    • Gelatinase beta antibody
    • GelatinaseB antibody
    • GELB antibody
    • Macrophage gelatinase antibody
    • MANDP2 antibody
    • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
    • Matrix Metalloproteinase 9 antibody
    • MMP 9 antibody
    • MMP-9 antibody
    • MMP9 antibody
    • MMP9_HUMAN antibody
    • Type V collagenase antibody
    see all

References for Anti-MMP9 antibody [2C3] (ab53296)

This product has been referenced in:
  • Hackel D  et al. Transient opening of the perineurial barrier for analgesic drug delivery. Proc Natl Acad Sci U S A 109:E2018-27 (2012). Rat . Read more (PubMed: 22733753) »
  • Radjabi AR  et al. Thrombin Induces Tumor Invasion through the Induction and Association of Matrix Metalloproteinase-9 and {beta}1-Integrin on the Cell Surface. J Biol Chem 283:2822-34 (2008). Inhibition Assay ; Human . Read more (PubMed: 18048360) »

See all 3 Publications for this product

Product Wall

I can confirm that ab53296 Anti-MMP9 antibody [2C3] antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concen...

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I confirmed with the lab and am happy to let you know that the datasheet is correct and this antibody does not contain any sodium azide nor does it contain any other preservative.

Thank you for your message. I notice from our records that you have been waiting a long time for a resonse and Isincerely apologise for the long delay. Iappreciate your patience.

I contacted the originator and regrettably they have taken a l...

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Thank you for your enquiry.

We do have a large range of isotype control antibodies which can be found on the following page from our website:


This includes several Mouse monoclonal...

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Thank you for your enquiry.

I can confirm that ab53296MMP9 antibody [2C3] is sold astissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration...

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