• Product name
    Anti-MMP9 antibody [2C3]
    See all MMP9 primary antibodies
  • Description
    Mouse monoclonal [2C3] to MMP9
  • Specificity
    ab53296 targets MMP9.
  • Tested applications
    Suitable for: WB, Inhibition Assay, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide:


    conjugated to Ovalbumin, corresponding to C terminal amino acids 603-614 of Human MMP9



Our Abpromise guarantee covers the use of ab53296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 78 kDa.
Inhibition Assay Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.


  • Function
    May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • Tissue specificity
    Produced by normal alveolar macrophages and granulocytes.
  • Involvement in disease
    Intervertebral disc disease
    Metaphyseal anadysplasia 2
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • Cellular localization
    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 82 kDa matrix metalloproteinase-9 antibody
    • 92 kDa gelatinase antibody
    • 92 kDa type IV collagenase antibody
    • CLG 4B antibody
    • CLG4B antibody
    • Collagenase Type 4 beta antibody
    • Collagenase type IV 92 KD antibody
    • EC antibody
    • Gelatinase 92 KD antibody
    • Gelatinase B antibody
    • Gelatinase beta antibody
    • GelatinaseB antibody
    • GELB antibody
    • Macrophage gelatinase antibody
    • MANDP2 antibody
    • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
    • Matrix Metalloproteinase 9 antibody
    • MMP 9 antibody
    • MMP-9 antibody
    • MMP9 antibody
    • MMP9_HUMAN antibody
    • Type V collagenase antibody
    see all

References for Anti-MMP9 antibody [2C3] (ab53296)

This product has been referenced in:
  • Hackel D  et al. Transient opening of the perineurial barrier for analgesic drug delivery. Proc Natl Acad Sci U S A 109:E2018-27 (2012). Rat . Read more (PubMed: 22733753) »
  • Radjabi AR  et al. Thrombin Induces Tumor Invasion through the Induction and Association of Matrix Metalloproteinase-9 and {beta}1-Integrin on the Cell Surface. J Biol Chem 283:2822-34 (2008). Inhibition Assay ; Human . Read more (PubMed: 18048360) »

See all 3 Publications for this product

Product Wall

Product Anti-MMP9 antibody [2C3] (ab53296) is provided in a Tissue Culture Supernatant (TCS) format; unfortunately, concentration information for this product is not available.

Antibody concentration is commonly determined by protein assay. A...

Read More

Product Anti-MMP9 antibody [2C3] (ab53296) is provided in a Tissue Culture Supernatant (TCS) format, and does not include sodium azide.


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