Overview

  • Product name
    Anti-MMP9 antibody [56-2A4]
    See all MMP9 primary antibodies
  • Description
    Mouse monoclonal [56-2A4] to MMP9
  • Specificity
    Recognizes the ~92 kDa latent and the ~83 kDa active forms of MMP9. Does not react with MMP1, MMP2, MMP3 or MMP13. The antibody recognizes precursor (92 kDa) and active (83 kDa) forms of human and guinea pig MMP-9.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Guinea pig, Human
  • Immunogen

    Synthetic peptide corresponding to Human MMP9 aa 626-644.
    Sequence:

    LFSGRRLWRFDVKAQMVDP


    Database link: P14780

  • Positive control
    • Breast carcinoma tissue. Conditioned medium of human fibrosarcoma HT1080 cells The protein is secreted so you may need to concentrate the protein from cell culture media. Concentrate protein by TCA, acetone or ammonium sulphate precipitation. HL60 cell lysate, U937 cell lysate, HT1080 cell lysate, human spleen lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab58803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 22496779
WB Use a concentration of 2 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 78 kDa).
IHC-Fr 1/500.

Target

  • Function
    May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • Tissue specificity
    Produced by normal alveolar macrophages and granulocytes.
  • Involvement in disease
    Intervertebral disc disease
    Metaphyseal anadysplasia 2
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • Cellular localization
    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 82 kDa matrix metalloproteinase-9 antibody
    • 92 kDa gelatinase antibody
    • 92 kDa type IV collagenase antibody
    • CLG 4B antibody
    • CLG4B antibody
    • Collagenase Type 4 beta antibody
    • Collagenase type IV 92 KD antibody
    • EC 3.4.24.35 antibody
    • Gelatinase 92 KD antibody
    • Gelatinase B antibody
    • Gelatinase beta antibody
    • GelatinaseB antibody
    • GELB antibody
    • Macrophage gelatinase antibody
    • MANDP2 antibody
    • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
    • Matrix Metalloproteinase 9 antibody
    • MMP 9 antibody
    • MMP-9 antibody
    • MMP9 antibody
    • MMP9_HUMAN antibody
    • Type V collagenase antibody
    see all

Anti-MMP9 antibody [56-2A4] images

  • Immunohistochemical analysis of rat aorta tissue, staining MMP9 with ab58803 at 1/100 dilution. Staining was detected using DAB.


  • Predicted band size : 78 kDa

    1:500 dilution of ab58803 detected MMP-9 on 10 µg of Human spleen lysates. Arrow indicates protein MMP-9 (~92 kDa). Lower bands indicate active forms of MMP-9

  • Immunocytochemical analysis of Human PANC-1 cells, labeling MMP9 with ab58803 (1/100). Cells were fixed in formaldehyde, permeabilized in 0.25% Triton-X100 and blocked in 1% BSA for 1 hour at 20°C. DAPI used to stain nuclei. 

    See Abreview

  • ab58803 staining MMP9 in murine rib sections by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and permeabilized using 0.1% Triton. Samples were then blocked with 20% serum for 1 hour at 19°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa Fluor®488-conjugated donkey anti-mouse polyclonal (green)  (ab150105) was used as secondary antibody at a 1/200 dilution. Counterstain DAPI (blue).

    See Abreview

  • Immunohistochemical analysis of Rabbit spleen tissue section, labeling MMP9 with ab58803 (1/50 with PBS and 0.05% Tween-20 at 23°C for 1 hour). Heat mediated antigen retrieval, using 10mM Citrate pH6. 

    See Abreview

  • Immunofluorescence analysis of murine neural stem cells, staining MMP9 (red) with ab58803. Cells were fixed in paraformaldehyde and blocked and permeabilized with 0.1% Triton-X and 10% goat serum. Cells were incubated with primary antibody and an AlexaFluor®568-conjugated goat anti-mouse IgG (ab175473) was used as the secondary antibody.

References for Anti-MMP9 antibody [56-2A4] (ab58803)

This product has been referenced in:
  • Chen Z  et al. Suppressive effect of formononetin on platelet-derived growth factor-BB-stimulated proliferation and migration of vascular smooth muscle cells. Exp Ther Med 12:1901-1907 (2016). Human . Read more (PubMed: 27588108) »
  • Lu X  et al. Evaluation of MMP-9 and MMP-2 and their suppressor TIMP-1 and TIMP-2 in adenocarcinoma of esophagogastric junction. Onco Targets Ther 9:4343-9 (2016). IHC . Read more (PubMed: 27486337) »

See all 21 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Citrate Buffer (pH=6)
Permeabilization
Yes - 0.1% Triton X-100 for 15 mins
Specification
Spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 20 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Panc1 cell line)
Permeabilization
Yes - 0.25% Triton-X100
Specification
Panc1 cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 15 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Rat Brain section from frontal cortex)
Specification
Rat Brain section from frontal cortex
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (ovaries)
Loading amount
50 µg
Specification
ovaries
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Mrs. Henda Nabli

Verified customer

Submitted Jul 17 2012

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (retina)
Specification
retina
Fixative
Paraformaldehyde
Permeabilization
Yes - Target Retrieval (Dako) for 20' at 95°C, followed by 20' cooldown at room temperature
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jul 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (uterus)
Specification
uterus
Fixative
10% buffered formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer at 95oC for 40 min
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Mrs. Henda Nabli

Verified customer

Submitted Jan 27 2012

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Rib)
Specification
Rib
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C
Username

Abcam user community

Verified customer

Submitted Jan 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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