• Product nameAnti-MMP9 antibody [56-2A4]
    See all MMP9 primary antibodies
  • Description
    Mouse monoclonal [56-2A4] to MMP9
  • SpecificityRecognizes the ~92 kDa latent and the ~83 kDa active forms of MMP9. Does not react with MMP1, MMP2, MMP3 or MMP13. The antibody recognizes precursor (92 kDa) and active (83 kDa) forms of human and guinea pig MMP-9.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Guinea pig, Human
  • Immunogen

    Synthetic peptide corresponding to Human MMP9 aa 626-644.


    Database link: P14780

  • Positive control
    • Breast carcinoma tissue. Conditioned medium of human fibrosarcoma HT1080 cells The protein is secreted so you may need to concentrate the protein from cell culture media. Concentrate protein by TCA, acetone or ammonium sulphate precipitation. HL60 cell lysate, U937 cell lysate, HT1080 cell lysate, human spleen lysate



Our Abpromise guarantee covers the use of ab58803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 22496779
WB Use a concentration of 2 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 78 kDa).
IHC-Fr 1/500.


  • FunctionMay play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • Tissue specificityProduced by normal alveolar macrophages and granulocytes.
  • Involvement in diseaseIntervertebral disc disease
    Metaphyseal anadysplasia 2
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • Cellular localizationSecreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 82 kDa matrix metalloproteinase-9 antibody
    • 92 kDa gelatinase antibody
    • 92 kDa type IV collagenase antibody
    • CLG 4B antibody
    • CLG4B antibody
    • Collagenase Type 4 beta antibody
    • Collagenase type IV 92 KD antibody
    • EC antibody
    • Gelatinase 92 KD antibody
    • Gelatinase B antibody
    • Gelatinase beta antibody
    • GelatinaseB antibody
    • GELB antibody
    • Macrophage gelatinase antibody
    • MANDP2 antibody
    • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
    • Matrix Metalloproteinase 9 antibody
    • MMP 9 antibody
    • MMP-9 antibody
    • MMP9 antibody
    • MMP9_HUMAN antibody
    • Type V collagenase antibody
    see all

Anti-MMP9 antibody [56-2A4] images

  • Immunohistochemical analysis of rat aorta tissue, staining MMP9 with ab58803 at 1/100 dilution. Staining was detected using DAB.

  • Predicted band size : 78 kDa

    1:500 dilution of ab58803 detected MMP-9 on 10 µg of Human spleen lysates. Arrow indicates protein MMP-9 (~92 kDa). Lower bands indicate active forms of MMP-9

  • Immunocytochemical analysis of Human PANC-1 cells, labeling MMP9 with ab58803 (1/100). Cells were fixed in formaldehyde, permeabilized in 0.25% Triton-X100 and blocked in 1% BSA for 1 hour at 20°C. DAPI used to stain nuclei. 

    See Abreview

  • ab58803 staining MMP9 in murine rib sections by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and permeabilized using 0.1% Triton. Samples were then blocked with 20% serum for 1 hour at 19°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa Fluor®488-conjugated donkey anti-mouse polyclonal (green)  (ab150105) was used as secondary antibody at a 1/200 dilution. Counterstain DAPI (blue).

    See Abreview

  • Immunohistochemical analysis of Rabbit spleen tissue section, labeling MMP9 with ab58803 (1/50 with PBS and 0.05% Tween-20 at 23°C for 1 hour). Heat mediated antigen retrieval, using 10mM Citrate pH6. 

    See Abreview

  • Immunofluorescence analysis of murine neural stem cells, staining MMP9 (red) with ab58803. Cells were fixed in paraformaldehyde and blocked and permeabilized with 0.1% Triton-X and 10% goat serum. Cells were incubated with primary antibody and an AlexaFluor®568-conjugated goat anti-mouse IgG (ab175473) was used as the secondary antibody.

References for Anti-MMP9 antibody [56-2A4] (ab58803)

This product has been referenced in:
  • Chen Z  et al. Suppressive effect of formononetin on platelet-derived growth factor-BB-stimulated proliferation and migration of vascular smooth muscle cells. Exp Ther Med 12:1901-1907 (2016). Human . Read more (PubMed: 27588108) »
  • De Groef L  et al. Decreased TNF Levels and Improved Retinal Ganglion Cell Survival in MMP-2 Null Mice Suggest a Role for MMP-2 as TNF Sheddase. Mediators Inflamm 2015:108617 (2015). IHC ; Mouse . Read more (PubMed: 26451076) »

See all 20 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rabbit Tissue sections (Spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM Citrate Buffer (pH=6)
Permeabilization Yes - 0.1% Triton X-100 for 15 mins
Specification Spleen
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 20 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Panc1 cell line)
Permeabilization Yes - 0.25% Triton-X100
Specification Panc1 cell line
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Jun 15 2016

Thank you for your phone call from last Friday.

We do have currently the ab34100 against IL8 which has been tested against rabbit protein and in ELISA. http://www.abcam.com/index.html?datasheet=34100 (or use the following: http://www.abcam.co...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Rat Brain section from frontal cortex)
Specification Rat Brain section from frontal cortex
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 07 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (ovaries)
Loading amount 50 µg
Specification ovaries
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Mrs. Henda Nabli

Verified customer

Submitted Jul 17 2012

Thank you very much for getting back to me. I hope you had a nice vacation.
Could you tell me about the image that was attached? What kind of samples are in each lane, and what are the molecular weights of the bands? Was this done using post-IP sa...

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Merci pour cette confirmation.

Comme convenu j'aimis en place l'envoi de 2 tubes de remplacement pour les ab1827 et ab58803. Le numéro de commande de remplacement gratuit de ce produit est ******. Vous recevrez prochainement un mail de ...

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Merci pour ces précisions.

Après vérification de notre stock,je peux égalementvous envoyer un lot différent pour ab1827 (TIMP1).

Merci de me confirmer que cette résolution vous convient et jep...

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Merci de nous avoir contactés.
Je suis désolé d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec l'anti-MMP9 ab58803 et l'anti-TIMP1 dont je vous demanderai de vérifier la ...

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Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (retina)
Specification retina
Fixative Paraformaldehyde
Permeabilization Yes - Target Retrieval (Dako) for 20' at 95°C, followed by 20' cooldown at room temperature
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 03 2012

1-10 of 20 Abreviews or Q&A