SpecificityRecognizes the ~92 kDa latent and the ~83 kDa active forms of MMP9.
Does not react with MMP1, MMP2, MMP3 or MMP13.
The antibody recognizes precursor (92 kDa) and active (83 kDa) forms of human and guinea pig MMP-9.
Breast carcinoma tissue.
Conditioned medium of human fibrosarcoma HT1080 cells
The protein is secreted so you may need to concentrate the protein from cell culture media. Concentrate protein by TCA, acetone or ammonium sulphate precipitation.
HL60 cell lysate, U937 cell lysate, HT1080 cell lysate, human spleen lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml.
Use at an assay dependent concentration. PubMed: 22496779
Use a concentration of 2 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 78 kDa).
FunctionMay play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Tissue specificityProduced by normal alveolar macrophages and granulocytes.
Involvement in diseaseIntervertebral disc disease Metaphyseal anadysplasia 2
Sequence similaritiesBelongs to the peptidase M10A family. Contains 3 fibronectin type-II domains. Contains 4 hemopexin repeats.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational modificationsProcessing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. N- and O-glycosylated.
Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
Matrix Metalloproteinase 9 antibody
MMP 9 antibody
Type V collagenase antibody
Anti-MMP9 antibody [56-2A4] images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [56-2A4] (ab58803)Image from Shang T et al., Journal of Surgical Research, Volume 178, Issue 2, December 2012, Pages 1029–1037. Fig 3.; doi: 10.1016/j.jss.2012.04.068. with permission from Elsevier.
Immunohistochemical analysis of rat aorta tissue, staining MMP9 with ab58803 at 1/100 dilution. Staining was detected using DAB.
Western blot - Anti-MMP9 [56-2A4] antibody (ab58803)
Predicted band size : 78 kDa
1:500 dilution of ab58803 detected MMP-9 on 10 µg of Human spleen lysates. Arrow indicates protein MMP-9 (~92 kDa). Lower bands indicate active forms of MMP-9
Immunocytochemistry/ Immunofluorescence - Anti-MMP9 antibody [56-2A4] (ab58803)This image is courtesy of an anonymous Abreview.
Immunocytochemical analysis of Human PANC-1 cells, labeling MMP9 with ab58803 (1/100). Cells were fixed in formaldehyde, permeabilized in 0.25% Triton-X100 and blocked in 1% BSA for 1 hour at 20°C. DAPI used to stain nuclei.
Immunohistochemistry (Frozen sections) - MMP9 antibody [56-2A4] (ab58803)Image courtesy of an anonymous Abreview.
ab58803 staining MMP9 in murine rib sections by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and permeabilized using 0.1% Triton. Samples were then blocked with 20% serum for 1 hour at 19°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa Fluor®488-conjugated donkey anti-mouse polyclonal (green) (ab150105) was used as secondary antibody at a 1/200 dilution. Counterstain DAPI (blue).
Immunocytochemistry/ Immunofluorescence - Anti-MMP9 antibody [56-2A4] (ab58803)Image from Njie eG et al., PLoS One. 2012;7(4):e34097. Epub 2012 Apr 4. Fig 2.; doi:10.1371/journal.pone.0034097; April 4, 2012, PLoS ONE 7(4): e34097.
Immunofluorescence analysis of murine neural stem cells, staining MMP9 (red) with ab58803. Cells were fixed in paraformaldehyde and blocked and permeabilized with 0.1% Triton-X and 10% goat serum. Cells were incubated with primary antibody and an AlexaFluor®568-conjugated goat anti-mouse IgG (ab175473) was used as the secondary antibody.
References for Anti-MMP9 antibody [56-2A4] (ab58803)
This product has been referenced in:
Chen Z et al. Suppressive effect of formononetin on platelet-derived growth factor-BB-stimulated proliferation and migration of vascular smooth muscle cells. Exp Ther Med12:1901-1907 (2016).
Read more (PubMed: 27588108) »
Lu X et al. Evaluation of MMP-9 and MMP-2 and their suppressor TIMP-1 and TIMP-2 in adenocarcinoma of esophagogastric junction. Onco Targets Ther9:4343-9 (2016).
Read more (PubMed: 27486337) »