Synthetic peptide corresponding to Human MMP9 aa 626-644.
Our Abpromise guarantee covers the use of ab58803 in the following tested applications.
|IHC-P||Use a concentration of 2 µg/ml.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22496779|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 78 kDa).|
1:500 dilution of ab58803 detected MMP-9 on 10 µg of Human spleen lysates. Arrow indicates protein MMP-9 (~92 kDa). Lower bands indicate active forms of MMP-9
Immunofluorescence analysis of murine neural stem cells, staining MMP9 (red) with ab58803. Cells were fixed in paraformaldehyde and blocked and permeabilized with 0.1% Triton-X and 10% goat serum. Cells were incubated with primary antibody and an AlexaFluor®568-conjugated goat anti-mouse IgG (ab175473) was used as the secondary antibody.
ab58803 staining MMP9 in murine rib sections by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and permeabilized using 0.1% Triton. Samples were then blocked with 20% serum for 1 hour at 19°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa Fluor®488-conjugated donkey anti-mouse polyclonal (green) (ab150105) was used as secondary antibody at a 1/200 dilution. Counterstain DAPI (blue).