Overview

  • Product nameAnti-MMP9 antibody [5G3]
    See all MMP9 primary antibodies
  • Description
    Mouse monoclonal [5G3] to MMP9
  • Tested applicationsSuitable for: WB, ICC, IHC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Monkey, Marmoset (common)
  • Immunogen

    Purified recombinant fragment of Human MMP9 expressed in E. Coli.

  • Positive control
    • This antibody gave a positive signal in WB with the following lysates: Recombinant protein; MMP9-hIgGFc transfected HEK293 cell lysate; U937; Human tonsil

Applications

Our Abpromise guarantee covers the use of ab119906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000.
ICC 1/200 - 1/1000.
IHC 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

Target

  • FunctionMay play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • Tissue specificityProduced by normal alveolar macrophages and granulocytes.
  • Involvement in diseaseIntervertebral disc disease
    Metaphyseal anadysplasia 2
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • Cellular localizationSecreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 82 kDa matrix metalloproteinase-9 antibody
    • 92 kDa gelatinase antibody
    • 92 kDa type IV collagenase antibody
    • CLG 4B antibody
    • CLG4B antibody
    • Collagenase Type 4 beta antibody
    • Collagenase type IV 92 KD antibody
    • EC 3.4.24.35 antibody
    • Gelatinase 92 KD antibody
    • Gelatinase B antibody
    • Gelatinase beta antibody
    • GelatinaseB antibody
    • GELB antibody
    • Macrophage gelatinase antibody
    • MANDP2 antibody
    • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
    • Matrix Metalloproteinase 9 antibody
    • MMP 9 antibody
    • MMP-9 antibody
    • MMP9 antibody
    • MMP9_HUMAN antibody
    • Type V collagenase antibody
    see all

Anti-MMP9 antibody [5G3] images

  • All lanes : Anti-MMP9 antibody [5G3] (ab119906) at 1/10000 dilution

    Lane 1 : Jurkat WCL (negative control)
    Lane 2 : Molt-4 WCL (negative control)
    Lane 3 : U937 WCL (positive control)
    Lane 4 : Human Tonsil (positive control)

    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 82,92 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 seconds
  • Anti-MMP9 antibody [5G3] (ab119906) at 1/500 dilution + recombinant Human MMP9 protein (238-465 amino acids)Predicted band size : 50.6 kDa
  • All lanes : Anti-MMP9 antibody [5G3] (ab119906) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : MMP7-hIgGFc transfected HEK293 cell lysate
    Lane 3 : MMP9-hIgGFc transfected HEK293 cell lysate
    Predicted band size : 50.6 kDa
  • Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

References for Anti-MMP9 antibody [5G3] (ab119906)

This product has been referenced in:
  • Wang TC  et al. Characterization of highly proliferative secondary tumor clusters along host blood vessels in malignant glioma. Mol Med Rep 12:6435-44 (2015). WB ; Rat . Read more (PubMed: 26299849) »
  • Yang Y  et al. Increased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasion. J Perinatol N/A:N/A (2014). Read more (PubMed: 25078863) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A549)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 60 µg
Specification A549
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Apr 06 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Marmoset (common) Tissue sections (Spleen)
Specification Spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Feb 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Monkey Tissue sections (Spleen)
Specification Spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Feb 08 2013

Thank you for contacting us.

This antibody has not been purified and therefore its concentration has not been determined. However, we estimate that as it is an ascites, it will have a concentration of approximately 5-10 mg/ml.

I h...

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Thank you very much for your call today, and I'm sorry for the trouble with these antibodies.

I'm sending free of charge vials of ab113697 and ab76003 on the order ***, which should arrive tomorrow.

Please keep me updated about how ...

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Thank you for your reply.

As requested I am sending you a vial of ab76003 and you should have the antibody tomorrow morning. For your records your new order number is *****.

If you have any issues with the new antibody or if there is ...

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Thank you for your reply and for providing more images of the results that you are getting whilst using this antibody.

I think the best way forward is to go ahead and either replace or refund the cost of the antibody, one potential replacement...

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Thank you for your reply.


I wonder if it is possible to also provide an image of your blot? It seems that it is possible that due to glycosylation, the MMP9 bands can easily be shifted to the MW you describe. Have you also tested another ...

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Thank you for contacting Abcam.

I am sorry about the issues that you are having with ab119903, Anti-MMP9 antibody [5G3] in western blot. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on r...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Jun 26 2012

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"