Overview

  • Product nameAnti-MMP9 antibody
    See all MMP9 primary antibodies
  • Description
    Rabbit polyclonal to MMP9
  • SpecificityThe antibody binds to Gelatinase-B, but does not cross react with the other MMP family members (MMP-1, MMP-2, MMP-3).
  • Tested applicationsIHC-P, IHC-Fr, WB, IP, ELISA, ICC/IF, ICC, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Full length MMP-9 native protein. (Mouse)

  • Positive control
    • HL60 cell lysate. U937 cell lysate. HT1080 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab38898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/1000. (see Abreview submitted by Greg Gibson)
WB 1/1000. Detects a band of approximately 92 kDa. When using colorimetric substrates such as BCIP/NBT use at a 1/5000 dilution (for chemiluminescent substrates). Detects a band of approximately 92-95 kDa (pro-form) and 82kDa (active form) (Human samples). Mouse MMP9 is larger, and on SDS PAGE gels runs about 102-105 kDa. Dilution optimised using Chromogenic detection.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF 1/500.
ICC Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19295156

Target

  • FunctionMay play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • Tissue specificityProduced by normal alveolar macrophages and granulocytes.
  • Involvement in diseaseIntervertebral disc disease
    Metaphyseal anadysplasia 2
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 82 kDa matrix metalloproteinase-9 antibody
    • 92 kDa gelatinase antibody
    • 92 kDa type IV collagenase antibody
    • CLG 4B antibody
    • CLG 4B antibody
    • CLG4B antibody
    • Collagenase Type 4 beta antibody
    • Collagenase Type 4 beta antibody
    • Collagenase type IV 92 KD antibody
    • Collagenase type IV 92 KD antibody
    • EC 3.4.24.35 antibody
    • Gelatinase 92 KD antibody
    • Gelatinase 92 KD antibody
    • Gelatinase B antibody
    • Gelatinase beta antibody
    • Gelatinase beta antibody
    • GelatinaseB antibody
    • GELB antibody
    • GELB antibody
    • Macrophage gelatinase antibody
    • Macrophage gelatinase antibody
    • MANDP2 antibody
    • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
    • Matrix Metalloproteinase 9 antibody
    • Matrix Metalloproteinase 9 antibody
    • MMP 9 antibody
    • MMP-9 antibody
    • MMP9 antibody
    • MMP9_HUMAN antibody
    • Type V collagenase antibody
    see all

Anti-MMP9 antibody images

  • Anti-MMP9 antibody (ab38898) + Human MMP9

    Observed band size : 88,92 kDa (why is the actual band size different from the predicted?)
    Ab38898 detects a band at 92 Kd (pro-form) and a band at 88 Kd (active form). Mouse MMP9 is slightly larger than human MMP9, and the antibody detects a band at about 105 Kd. It is recommend to concentrate samples by Gelatin-agarose affinity chromatography prior to Western blot usage. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples.
  • All lanes : Anti-MMP9 antibody (ab38898) at 1/1000 dilution

    Lane 1 : Human lung tissue lysate at 100 µg with 10% Milk for 1 hour at room temperature
    Lane 2 : Human lung tissue lysate at 100 µg with 10% Milk for 1 hour at room temperature
    Lane 3 : MMP-9 KO mice tissue lysates with 10% Milk for 1 hour at room temperature
    Lane 4 : MMP-9 KO mice tissue lysates with 10% Milk for 1 hour at room temperature

    Secondary
    HRP-conjugated donkey anti-rabbit polyclonal at 1/1000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    This image is courtesy of an anonymous Abreview

    Specific observed bands 95-100 kDa

    See Abreview

  • ab38898 at a 1/1000 dilution staining mouse heart tissue by Immunohistochemistry (Frozen sections). The tissue was removed from a mouse, rinsed in PBS and slowly frozen in supercooled isopentane. 14um sections were made using a cryostat. The sections were acetone fixed and blocked in 2% BSA prior to incubation with the MMP9 antibody. A Cy3 conjugated goat anti-rabbit antibody (ab6939) was used as the secondary antibody. In the image: red staining = MMP9, blue staining = nuclei, green = gelatinase activity.

  • ab38898 staining MMP9 in Mouse Pancreatic carcinoma tissue sections by IHC-P (formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 1 hour at room temperature. Antigen retrieval was by heat mediation in citric acid (pH6). Samples were incubated with primary antibody (1/100) in 1% Aurion BSA for 12 hours. An HRP-conjugated Donkey polyclonal to rabbit IgG (1/100) was used as secondary antibody.

    See Abreview

  • ab38898 staining MMP9 in 6 month-old transgenic zebrafish pancreas (Ihha overexpression) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Sections were incubated with primary antibody (1/500) and HRP-conjugated secondary antibody colored using DAB solution. Slides were counterstained with hematoxylin.

References for Anti-MMP9 antibody (ab38898)

This product has been referenced in:
  • Kothari P  et al. IL-6-mediated induction of matrix metalloproteinase-9 is modulated by JAK-dependent IL-10 expression in macrophages. J Immunol 192:349-57 (2014). WB . Read more (PubMed: 24285838) »
  • Przybyla BD  et al. Molecular changes in bone marrow, tumor and serum after conductive ablation of murine 4T1 breast carcinoma. Int J Oncol 44:600-8 (2014). WB ; Mouse . Read more (PubMed: 24270800) »

See all 57 Publications for this product

Product Wall

Abreviews
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Mouse Tissue lysate - whole (mouse brain (cortex))
Specification mouse brain (cortex)
Blocking step ECL Blocking Agent (RPN 2125, GE Healthcare) as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Jeroen Aerts

Verified customer

Submitted Aug 01 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (Follicle)
Specification Follicle
Permeabilization No
Fixative Formaldehyde
Username

Ms. Jaeeun Jung

Verified customer

Submitted Jul 07 2014

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Neutrophils and Monocytes)
Specification Neutrophils and Monocytes
Permeabilization Yes - 2%BSA + 0.2% tritonX100 in PBS
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 13 2014

Application Western blot
Loading amount 100000 cells
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Jurkat)
Specification Jurkat
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Feb 13 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (tooth)
Specification tooth
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (stomach cancer cell)
Loading amount 30 µg
Specification stomach cancer cell
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Melanoma B16F10 tumor)
Specification Melanoma B16F10 tumor
Fixative 4% Formalin
Permeabilization Yes - 0.1% SDS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1%
Username

Ms. Seontae Kim

Verified customer

Submitted Feb 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Melanoma cancer)
Loading amount 30 µg
Specification Melanoma cancer
Treatment 5gy Irradiation
Gel Running Conditions Non-reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Ms. Seontae Kim

Verified customer

Submitted Jan 08 2013

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