The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
1/50 - 1/100.
FunctionMay play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Tissue specificityProduced by normal alveolar macrophages and granulocytes.
Involvement in diseaseIntervertebral disc disease Metaphyseal anadysplasia 2
Sequence similaritiesBelongs to the peptidase M10A family. Contains 3 fibronectin type-II domains. Contains 4 hemopexin repeats.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational modificationsProcessing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. N- and O-glycosylated.
Immunohistochemical analysis of paraffin-embedded
human breast carcinoma tissue using MMP9 antibody ab53018 1/50. The left hand image shows untreated tissue; right hand image shows tissue treated with the immunising peptide.
Western blot - MMP9 antibody (ab53018)
All lanes : Anti-MMP9 antibody (ab53018) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells with no immunizing peptide Lane 2 : Extracts from HepG2 cells with immunizing peptide
Predicted band size : 78 kDa Observed band size : 78 kDa
References for Anti-MMP9 antibody (ab53018)
has not yet been referenced specifically in any publications.
Publishing research using ab53018? Please let us know so that we can cite the reference in this datasheet.
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