Recombinant Anti-MMP9 antibody [EP1255Y] (ab137867)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1255Y] to MMP9
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MMP9 antibody [EP1255Y]
See all MMP9 primary antibodies -
Description
Rabbit monoclonal [EP1255Y] to MMP9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MMP9 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: U937, A549 (Human lung carcinoma epithelial cell) serum starved overnight, then treated with 80nM TPA for 24 hours whole cell lysate and MDA-MB-231 (Human breast adenocarcinoma epithelial cell) serum starved overnight, then treated with 200nM TPA for 24 hours whole cell lysate IHC-P: Human tonsil and lymphoma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1255Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (5) |
1/1000. Predicted molecular weight: 78 kDa.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/800. |
Notes |
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WB
1/1000. Predicted molecular weight: 78 kDa. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/800. |
Target
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Function
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. -
Tissue specificity
Produced by normal alveolar macrophages and granulocytes. -
Involvement in disease
Intervertebral disc disease
Metaphyseal anadysplasia 2 -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsProcessing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 4318 Human
- Omim: 120361 Human
- SwissProt: P14780 Human
- Unigene: 297413 Human
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Alternative names
- 82 kDa matrix metalloproteinase-9 antibody
- 92 kDa gelatinase antibody
- 92 kDa type IV collagenase antibody
see all
Images
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All lanes : Anti-MMP9 antibody [EP1255Y] (ab137867) at 1/1000 dilution
Lane 1 : LoVo (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 6 : A549 (Human lung carcinoma epithelial cell) serum starved overnight whole cell lysate
Lane 7 : A549 (Human lung carcinoma epithelial cell) serum starved overnight, then treated with 80nM TPA for 24 hours whole cell lysate
Lane 8 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) serum starved overnight whole cell lysate
Lane 9 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) serum starved overnight, then treated with 200nM TPA for 24 hours whole cell lysate
Lane 10 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 78 kDa
Observed band size: 84 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking buffer and concentration 5% NFDM/TBST
Diluting buffer and concentration 5% NFDM/TBSTThe expression of MMP-9 can be stimulated by various agents, such as inflammatory cytokine, growth factor, and 12-O-tetradecanoylphorbol-13-acetate (TPA) (PMID:21047770, 28969043).
Treatment increasing the expression of MMP-9 is recommended when using this antibody.
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Anti-MMP9 antibody [EP1255Y] (ab137867) at 1/1000 dilution (unpurified) + U937 cell lysate at 10 µg
Secondary
Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling MMP9 with unpurified ab137867 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling MMP9 with purified ab137867 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma tissue labelling MMP9 with unpurified ab137867 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (102)
ab137867 has been referenced in 102 publications.
- Wu R et al. Hsa_circ_0003602 Contributes to the Progression of Colorectal Cancer by Mediating the miR-149-5p/SLC38A1 Axis. Gut Liver 17:267-279 (2023). PubMed: 36148577
- Tang J et al. Circ_0006220 Contributes to NSCLC Progression through miR-342-3p/GOT2 Axis. Ann Thorac Cardiovasc Surg 29:11-22 (2023). PubMed: 36575008
- Yin P et al. Chemoprevention of 4NQO-Induced Mouse Tongue Carcinogenesis by AKT Inhibitor through the MMP-9/RhoC Signaling Pathway and Autophagy. Anal Cell Pathol (Amst) 2022:3770715 (2022). PubMed: 36247874
- Kirkpatrick JD et al. Protease activity sensors enable real-time treatment response monitoring in lymphangioleiomyomatosis. Eur Respir J 59:N/A (2022). PubMed: 34561286
- Mamie C et al. MMP9 expression in intestinal fistula from patients with fistulizing CD and from human xenograft mouse model. Tissue Barriers 10:1994350 (2022). PubMed: 34709129