FunctionMay play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Tissue specificityProduced by normal alveolar macrophages and granulocytes.
Involvement in diseaseIntervertebral disc disease Metaphyseal anadysplasia 2
Sequence similaritiesBelongs to the peptidase M10A family. Contains 3 fibronectin type-II domains. Contains 4 hemopexin repeats.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational modificationsProcessing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. N- and O-glycosylated.
This image shows human colon tissue stained with ab51203 at 5µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP9 antibody [SB15c] (ab51203)Image from Stenvold H et al., Lung Cancer, Volume 75, Issue 2, February 2012, Pages 235–241. Fig 1.; doi: 10.1016/j.lungcan.2011.06.010. with permission from Elsevier.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human non-small-cell lung cancer tissue staining MMP9 with ab51203.
Antigen retrieval was performed by placing the sections in 0.01 M citrate buffer (pH 6) and heating in a microwave oven. Samples were incubated with primary antibody (1/100) overnight at 4°C. Staining was detected using an IHC substrate kit.
References for Anti-MMP9 antibody [SB15c] (ab51203)
This product has been referenced in:
Liu GH et al. MicroRNA-524-5p suppresses the growth and invasive abilities of gastric cancer cells. Oncol Lett11:1926-1932 (2016).
Read more (PubMed: 26998102) »
Stenvold H et al. Overexpression of matrix metalloproteinase-7 and -9 in NSCLC tumor and stromal cells: correlation with a favorable clinical outcome. Lung Cancer75:235-41 (2012).
Read more (PubMed: 21764478) »