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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP9 antibody - Whole molecule
See all MMP9 products (27) ...
Description
Rabbit polyclonal to MMP9 - Whole molecule
Specificity
The antibody binds to Gelatinase-B, but does not cross react with the other MMP family members (MMP-1, MMP-2, MMP-3).
Tested applications
IHC-P, IHC-Fr, WB, IP, ELISA, ICC/IF, ICC, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Dog, Human
Immunogen
Full length MMP-9 native protein. (Mouse)
Positive control
HL60 cell lysate. U937 cell lysate. HT1080 cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/1000.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/1000((see Abreview submitted by Greg Gibson))
WB: 1/1000Detects a band of approximately 92 kDa.(When using colorimetric substrates such as BCIP/NBT use at a 1/5000 dilution (for chemiluminescent substrates). Detects a band of approximately 92-95 kDa (pro-form) and 82kDa (active form) (Human samples). Mouse MMP9 is larger, and on SDS PAGE gels runs about 102-105 kDa. Dilution optimised using Chromogenic detection.)
IP: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent concentration.
ICC: Use at an assay dependent dilution.
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 19295156)
Target
Function
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Tissue specificity
Produced by normal alveolar macrophages and granulocytes.
Involvement in disease
Defects in MMP9 may be a cause of susceptibility to intervertebral disc disease (IDD) [MIM:603932]; also known as lumbar disk herniation (LDH). IDD is one of the most common musculo-skeletal disorders and the predominant cause of low-back pain and unilateral leg pain.
Defects in MMP9 are the cause of metaphyseal anadysplasia type 2 (MANDP2) [MIM:613073]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP14780
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 82 kDa matrix metalloproteinase-9 antibody
- 92 kDa gelatinase antibody
- 92 kDa type IV collagenase antibody
see all
Database links
- Entrez Gene: 403885 Dog
- Entrez Gene: 4318 Human
- Entrez Gene: 17395 Mouse
- Omim: 120361 Human
- SwissProt: O18733 Dog
- SwissProt: P14780 Human
- SwissProt: P41245 Mouse
- Unigene: 297413 Human
see all
Anti-MMP9 antibody - Whole molecule images:
Western blot - MMP9 antibody (ab38898)
Anti-MMP9 antibody - Whole molecule (ab38898) + Human MMP9
Observed band size : 88,92 kDa (why is the actual band size different from the predicted?)
Ab38898 detects a band at 92 Kd (pro-form) and a band at 88 Kd (active form). Mouse MMP9 is slightly larger than human MMP9, and the antibody detects a band at about 105 Kd.
It is recommend to concentrate samples by Gelatin-agarose affinity chromatography prior to Western blot usage. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples.
Immunohistochemistry (Frozen sections) - MMP9 antibody - Whole molecule (ab38898)
ab38898 at a 1/1000 dilution staining mouse heart tissue by Immunohistochemistry (Frozen sections). The tissue was removed from a mouse, rinsed in PBS and slowly frozen in supercooled isopentane. 14um sections were made using a cryostat. The sections were acetone fixed and blocked in 2% BSA prior to incubation with the MMP9 antibody. A Cy3 conjugated goat anti-rabbit antibody was used as the secondary antibody. In the image: red staining = MMP9, blue staining = nuclei, green = gelatinase activity.
Western blot - MMP9 antibody - Whole molecule (ab38898)
All lanes : Anti-MMP9 antibody - Whole molecule (ab38898) at 1/1000 dilution
Lane 1 : Human lung tissue lysate at 100 µg with 10% Milk for 1 hour at room temperature
Lane 2 : Human lung tissue lysate at 100 µg with 10% Milk for 1 hour at room temperature
Lane 3 : MMP-9 KO mice tissue lysates with 10% Milk for 1 hour at room temperature
Lane 4 : MMP-9 KO mice tissue lysates with 10% Milk for 1 hour at room temperature
Secondary
HRP-conjugated donkey anti-rabbit polyclonal at 1/1000 dilution
developed using the ECL technique
Performed under reducing conditions.
Specific observed bands 95-100 kDa
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP9 antibody - Whole molecule (ab38898)
ab38898 staining MMP9 in Mouse Pancreatic carcinoma tissue sections by IHC-P (formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 1 hour at room temperature. Antigen retrieval was by heat mediation in citric acid (pH6). Samples were incubated with primary antibody (1/100) in 1% Aurion BSA for 12 hours. An HRP-conjugated Donkey polyclonal to rabbit IgG (1/100) was used as secondary antibody.
This image is courtesy of an anonymous Abreview
Protocols
References for Anti-MMP9 antibody - Whole molecule (ab38898)
This product has been referenced in:
- Gruber Cet al. Spliceosome-mediated RNA trans-splicing facilitates targeted delivery of suicide genes to cancer cells. Mol Cancer Ther 10:233-41 (2011). WB; Human.Read more (PubMed: 21209069) »
- Ungvari Zet al. Adaptive induction of NF-E2-related factor-2-driven antioxidant genes in endothelial cells in response to hyperglycemia. Am J Physiol Heart Circ Physiol 300:H1133-40 (2011).Read more (PubMed: 21217061) »
See all 23 publications for this product
Publishing research using ab38898? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"