MMP9 protein (ab39309)

Product Name 

MMP9 protein

Product type 

Proteins and Peptides

Peptide 

Metalloproteinase purified from cell culture media of BHK cells transfected with Murine 105 kD Gelatinase (murine homologue of Human MMP9).

Specificity 

We have a range of domain specific antibodies for this target. For a full list please see all MMP9 antibodies

Tested applications 
(see key)

FuncS, SDS-PAGE, WB

Application notes 
(see key)

Recommended dilutions
SDS-PAGE: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. It runs at approximately 105 kDa. NOTE: The enzyme is supplied predominantly as a zymogen, with a small amount of active enzyme present. The zymogen can be activated by diluting the produt to 10 - 50 ng/ul in a buffer containing 50 mM Tris-HCl, 150 mM NaCl, 10 mM CaCl2, and 10 uM ZnCl2, pH 7.4. The product should be incubated at 37 C for 2-6 hours with the organomercurial compound APMA at 1 mM. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

Relevance 

All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn(2+) binding endopeptidases that degrade various components of the ECM. MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. These enzymes are very potent when active, and are associated with extracellular space inhibitors called TIMPs (tissue inhibitors of matrix metalloproteinases). TIMPs have been shown to block tumor cell invasion suggesting that they act as metastasis suppressor genes.

MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III and is produced by normal alveolar macrophages and granulocytes. The expression of MMP9 increases in Epstein-Barr virus infected lymphoma derived cell lines and may be of significance in typically invasive nasopharyngeal carcinomas. MMP9 is constitutively produced by some tumor cell lines (e.g.: HT1080, HL60, U937) but not by most quiescent cells and tissues. Treatment of cells with the phorbol ester TPA stimulates production of MMP9 in some cell types, but the low protein levels produced (pg/ml) often require concentration of cell culture media to visualize the bands by Western blotting.

Storage buffer 

Preservative: None
Constituents: 50% Glycerol, PBS, 250mM Sodium chloride, 0.6 % DMSO, pH 7.4

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Purification notes 

Purified from cell culture media of BHK cells, free of its endogenous inhibitor, TIMP1, and other matrix metalloproteinases.

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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