Overview

  • Product nameAnti-MMS2 antibody
    See all MMS2 primary antibodies
  • Description
    Rabbit polyclonal to MMS2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human MMS2.

  • Positive control
    • This antibody gave a positive signal in mouse testis tissue lysate and in the following whole cell lysates: HL60; HeLa; U20S; Jurkat; NIH3T3; PC12.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab101475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionHas no ubiquitin ligase activity on its own. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.
  • Tissue specificityDetected in placenta, colon, liver and skin. Detected at very low levels in most tissues.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 alpha 25 dihydroxyvitamin D3 inducible antibody
    • DDVit 1 antibody
    • DDVIT1 antibody
    • EDAF-1 antibody
    • EDAF1 antibody
    • EDPF-1 antibody
    • EDPF1 antibody
    • Enterocyte differentiation associated factor EDAF 1 antibody
    • Enterocyte differentiation promoting factor antibody
    • Enterocyte differentiation-associated factor 1 antibody
    • Enterocyte differentiation-promoting factor 1 antibody
    • Methyl methanesulfonate sensitive 2 antibody
    • MMS 2 antibody
    • MMS2 antibody
    • MMS2 homolog antibody
    • UB2V2_HUMAN antibody
    • UBE2V 2 antibody
    • UBE2V2 antibody
    • Ubiquitin conjugating enzyme E2 variant 2 antibody
    • Ubiquitin conjugating enzyme E2v2 antibody
    • Ubiquitin-conjugating enzyme E2 variant 2 antibody
    • UEV 2 antibody
    • UEV2 antibody
    • Vitamin D3 inducible protein antibody
    • Vitamin D3-inducible protein antibody
    see all

Anti-MMS2 antibody images

  • All lanes : Anti-MMS2 antibody (ab101475) at 1 µg/ml

    Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 16 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ICC/IF image of ab101475 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101475, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 5µg/ml.
  • All lanes : Anti-MMS2 antibody (ab101475) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : Testis (Mouse) Tissue Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 16 kDa
    Observed band size : 16 kDa
    Additional bands at : 74 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References for Anti-MMS2 antibody (ab101475)

ab101475 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Saccharomyces cerevisiae Cell lysate - whole cell (Yeast cell extract)
Specification Yeast cell extract
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Submitted Jan 13 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"