Overview

  • Product nameAnti-MNAT1 antibody
    See all MNAT1 primary antibodies
  • Description
    Rabbit polyclonal to MNAT1
  • SpecificityDetects endogenous levels of total MNAT1 protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Human) from an internal region

  • Positive control
    • Extracts from HeLa, HepG2 and 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab65125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ELISA 1/20000.

Target

  • FunctionStabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.
  • Tissue specificityHighest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes.
  • Sequence similaritiesContains 1 RING-type zinc finger.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MNAT 1 antibody
    • CAP35 antibody
    • CDK activating kinase assembly factor MAT1 antibody
    • CDK-activating kinase assembly factor MAT1 antibody
    • CDK7/cyclin H assembly factor antibody
    • CDK7/cyclin-H assembly factor antibody
    • Cyclin G1 interacting protein antibody
    • Cyclin-G1-interacting protein antibody
    • MAT1 antibody
    • MAT1_HUMAN antibody
    • Menage a trois 1 (CAK assembly factor) antibody
    • Menage a trois antibody
    • Menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis) antibody
    • MNAT CDK activating kinase assembly factor 1 antibody
    • Mnat1 antibody
    • p35 antibody
    • p36 antibody
    • RING finger protein 66 antibody
    • RING finger protein MAT1 antibody
    • RNF66 antibody
    • TFB3 antibody
    see all

Anti-MNAT1 antibody images

  • All lanes : Anti-MNAT1 antibody (ab65125) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HepG2 cell extract
    Lane 3 : 293 cell extract
    Lane 4 : 293 cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

References for Anti-MNAT1 antibody (ab65125)

ab65125 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"