Recombinant
RabMAb

Anti-MNAT1 antibody [EPR10940] (ab169546)

Overview

  • Product name
    Anti-MNAT1 antibody [EPR10940]
    See all MNAT1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10940] to MNAT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to residues in Human MNAT1 (UniProt P51948).

  • Positive control
    • HeLa, A431, 293T and HepG2 cell lysates; HepG2 cells; Immunoprecipitation pellet from HeLa cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 36 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.
    • Tissue specificity
      Highest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes.
    • Sequence similarities
      Contains 1 RING-type zinc finger.
      Contains 1 UIM (ubiquitin-interacting motif) repeat.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • MNAT 1 antibody
      • CAP35 antibody
      • CDK activating kinase assembly factor MAT1 antibody
      • CDK-activating kinase assembly factor MAT1 antibody
      • CDK7/cyclin H assembly factor antibody
      • CDK7/cyclin-H assembly factor antibody
      • Cyclin G1 interacting protein antibody
      • Cyclin-G1-interacting protein antibody
      • MAT1 antibody
      • MAT1_HUMAN antibody
      • Menage a trois 1 (CAK assembly factor) antibody
      • Menage a trois antibody
      • Menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis) antibody
      • MNAT CDK activating kinase assembly factor 1 antibody
      • Mnat1 antibody
      • p35 antibody
      • p36 antibody
      • RING finger protein 66 antibody
      • RING finger protein MAT1 antibody
      • RNF66 antibody
      • TFB3 antibody
      see all

    Images

    • All lanes : Anti-MNAT1 antibody [EPR10940] (ab169546) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 36 kDa

    • Anti-MNAT1 antibody [EPR10940] (ab169546) at 1/1000 dilution + immunoprecipitation pellet from HeLa cell lysate

      Predicted band size: 36 kDa

    • Immunofluorescent analysis of HepG2 cells labeling MNAT1 with ab169546 at 1/100 dilution.

    References

    ab169546 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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