Anti-MNAT1 antibody [EPR2554(2)] (ab169774)


  • Product name
    Anti-MNAT1 antibody [EPR2554(2)]
    See all MNAT1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2554(2)] to MNAT1
  • Host species
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MNAT1.
    Database link: P51948

  • Positive control
    • HeLa, HepG2, A431 and 293T cell lysates, HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab169774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.
    • Tissue specificity
      Highest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes.
    • Sequence similarities
      Contains 1 RING-type zinc finger.
      Contains 1 UIM (ubiquitin-interacting motif) repeat.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • MNAT 1 antibody
      • CAP35 antibody
      • CDK activating kinase assembly factor MAT1 antibody
      • CDK-activating kinase assembly factor MAT1 antibody
      • CDK7/cyclin H assembly factor antibody
      • CDK7/cyclin-H assembly factor antibody
      • Cyclin G1 interacting protein antibody
      • Cyclin-G1-interacting protein antibody
      • MAT1 antibody
      • MAT1_HUMAN antibody
      • Menage a trois 1 (CAK assembly factor) antibody
      • Menage a trois antibody
      • Menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis) antibody
      • MNAT CDK activating kinase assembly factor 1 antibody
      • Mnat1 antibody
      • p35 antibody
      • p36 antibody
      • RING finger protein 66 antibody
      • RING finger protein MAT1 antibody
      • RNF66 antibody
      • TFB3 antibody
      see all


    • Flow Cytometrical analysis of permeabilized HeLa cells with ab169774 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).

    • All lanes : Anti-MNAT1 antibody [EPR2554(2)] (ab169774) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 36 kDa
      Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.


    ab169774 has not yet been referenced specifically in any publications.

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