Recombinant
RabMAb

Anti-MNAT1 antibody [EPR2555(2)] (ab154802)

Overview

  • Product name
    Anti-MNAT1 antibody [EPR2555(2)]
    See all MNAT1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2555(2)] to MNAT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MNAT1. The exact sequence is proprietary.

  • Positive control
    • A431, HeLa, A673 and Raw264.7 cell lysates; HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.
    • Tissue specificity
      Highest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes.
    • Sequence similarities
      Contains 1 RING-type zinc finger.
      Contains 1 UIM (ubiquitin-interacting motif) repeat.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • MNAT 1 antibody
      • CAP35 antibody
      • CDK activating kinase assembly factor MAT1 antibody
      • CDK-activating kinase assembly factor MAT1 antibody
      • CDK7/cyclin H assembly factor antibody
      • CDK7/cyclin-H assembly factor antibody
      • Cyclin G1 interacting protein antibody
      • Cyclin-G1-interacting protein antibody
      • MAT1 antibody
      • MAT1_HUMAN antibody
      • Menage a trois 1 (CAK assembly factor) antibody
      • Menage a trois antibody
      • Menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis) antibody
      • MNAT CDK activating kinase assembly factor 1 antibody
      • Mnat1 antibody
      • p35 antibody
      • p36 antibody
      • RING finger protein 66 antibody
      • RING finger protein MAT1 antibody
      • RNF66 antibody
      • TFB3 antibody
      see all

    Images

    • All lanes : Anti-MNAT1 antibody [EPR2555(2)] (ab154802) at 1/1000 dilution

      Lane 1 : A431 lysate
      Lane 2 : HeLa lysate
      Lane 3 : A673 lysate
      Lane 4 : RAW264.7 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 44 kDa

    • Immunofluorescence analysis of HeLa cells labeling MAT1 with ab154802 at 1/100 dilution.
    • Flow cytometric analysis of permeabilized Hela cells labeling MAT1 with ab154802 at 1/10 dilution (red) or a negative rabbit IgG (green).

    References

    ab154802 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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