Overview

  • Product name
    Anti-MNDA antibody - N-terminal
    See all MNDA primary antibodies
  • Description
    Rabbit polyclonal to MNDA - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MNDA aa 33-61 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P41218

  • Positive control
    • CEM cell lysate; Human placenta tissue, CEM cells

Properties

Applications

Our Abpromise guarantee covers the use of ab170302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 46 kDa.

Target

  • Function
    May act as a transcriptional activator/repressor in the myeloid lineage. Plays a role in the granulocyte/monocyte cell-specific response to interferon. Stimulates the DNA binding of the transcriptional repressor protein YY1.
  • Tissue specificity
    Expressed constitutively in cells of the myeloid lineage. Found in promyelocyte stage cells as well as in all other stage cells including peripheral blood monocytes and granulocytes. Also appear in myeloblast cells in some cases of acute myeloid Leukemia.
  • Sequence similarities
    Contains 1 DAPIN domain.
    Contains 1 HIN-200 domain.
  • Domain
    Its N-terminal half (200 amino acids) is sufficient for maximum enhancement of YY1 DNA binding and a portion of this sequence is responsible for binding YY1.
  • Cellular localization
    Nucleus. Cytoplasm. Uniformly distributed throughout the interphase cell nucleus. Associates with chromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • MNDA antibody
    • MNDA_HUMAN antibody
    • Myeloid cell nuclear differentiation antigen antibody
    • OTTHUMP00000024384 antibody
    • PYHIN3 antibody
    see all

Images

  • All lanes : Anti-MNDA antibody - N-terminal (ab170302) at 1/100 dilution

    Lane 1 : CEM cell lysate
    Lane 2 : CEM cell lysate with blocking peptide

    Lysates/proteins at 12.5 µg per lane.

    Predicted band size: 46 kDa

  • Immuhistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling MNDA with ab170302 at 1/50 dilution.

  • Flow cytometric analysis of CEM cells labeling MNDA with ab170302 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram).

References

ab170302 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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