Overview

  • Product nameAnti-MNK1 antibody [2H8]
    See all MNK1 primary antibodies
  • Description
    Mouse monoclonal [2H8] to MNK1
  • Tested applicationsSuitable for: WB, Sandwich ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human MNK1 protein (NP_003675.2).

  • Positive control
    • Human tonsil tissue; HeLa cell lysate and Transfected 293T cell lysate; HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab117545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 51 kDa.
Sandwich ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionMay play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase.
  • Cellular localizationCytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MAP kinase interacting kinase 1 antibody
    • MAP kinase interacting serine/threonine kinase 1 antibody
    • MAP kinase signal integrating kinase 1 antibody
    • MAP kinase signal-integrating kinase 1 antibody
    • MAP kinase-interacting serine/threonine-protein kinase 1 antibody
    • MAPK signal integrating kinase 1 antibody
    • MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1 antibody
    • mknk1 antibody
    • MKNK1_HUMAN antibody
    • MNK 1 antibody
    • Mnk1 antibody
    see all

Anti-MNK1 antibody [2H8] images

  • ab115457, at 5 µg/ml, staining MNK1 in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.
  • ab117545 at 10µg/ml dilution staining MNK1 in HeLa cells by Immunofluorescence.
  • sELISA: Detection limit for recombinant proprietary tagged MNK1 is approximately 3ng/ml as a capture antibody (ab117545).
  • Anti-MNK1 antibody [2H8] (ab117545) + HeLa cell lysate

    Predicted band size : 51 kDa
  • All lanes : Anti-MNK1 antibody [2H8] (ab117545)

    Lane 1 : 293T cell lysate, transfected with MNK1
    Lane 2 : 293T cell lysate, non-transfected


    Predicted band size : 51 kDa

References for Anti-MNK1 antibody [2H8] (ab117545)

ab117545 has not yet been referenced specifically in any publications.

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