The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionMay play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Post-translational modificationsDual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase.
Cellular localizationCytoplasm and Cytoplasm. Nucleus.
Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using ab111292 at a dilution of 1/50. Left panel shows staining without immunizing peptide right panel shows staining with immunizing peptide
References for Anti-MNK1 antibody (ab111292)
has not yet been referenced specifically in any publications.
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