Overview

  • Product nameAnti-MNK1 antibody
    See all MNK1 primary antibodies
  • Description
    Mouse monoclonal to MNK1
  • Tested applicationsSuitable for: Flow Cyt, WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length human MNK1 protein (AAH02755) linked to a proprietary tag.

  • Positive control
    • WB: the immunogen; Raw 264.7 cell lysate IHC-P: human small intestine tissue ICC/IF: HeLa cell

Properties

Applications

Our Abpromise guarantee covers the use of ab89223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 51 kDa.
ELISA Use at an assay dependent concentration. The detection limit for recombinant tagged MNK1 is approximately 10 ng/ml when used as a capture antibody.
IHC-P Use a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionMay play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase.
  • Cellular localizationCytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MAP kinase interacting kinase 1 antibody
    • MAP kinase interacting serine/threonine kinase 1 antibody
    • MAP kinase signal integrating kinase 1 antibody
    • MAP kinase signal-integrating kinase 1 antibody
    • MAP kinase-interacting serine/threonine-protein kinase 1 antibody
    • MAPK signal integrating kinase 1 antibody
    • MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1 antibody
    • mknk1 antibody
    • MKNK1_HUMAN antibody
    • MNK 1 antibody
    • Mnk1 antibody
    see all

Anti-MNK1 antibody images

  • Anti-MNK1 antibody (ab89223) at 5 µg/ml + immunogen at 0.2 µg
    Developed using the ECL technique

    Predicted band size : 51 kDa
    Observed band size : 83 kDa (why is the actual band size different from the predicted?)
  • Anti-MNK1 antibody (ab89223) at 5 µg/ml + Raw 264.7 cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 51 kDa
    Observed band size : ~45 kDa (why is the actual band size different from the predicted?)
  • ab89223, at 3 µg/ml, staining MNK1 in formalin fixed, paraffin embedded human small intestine tissue by Immunoperoxidase/Immunohistochemistry.
  • ab89223, at 10 µg/ml, staining MNK1 in paraformaldehyde fixed, permeabilised HeLa cell by Immunofluorescence.
  • Overlay histogram showing HeLa cells stained with ab89223 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab89223, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Anti-MNK1 antibody (ab89223)

ab89223 has not yet been referenced specifically in any publications.

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