Anti-MNK1 (phospho T250) antibody (ab111434)

Overview

  • Product nameAnti-MNK1 (phospho T250) antibody
    See all MNK1 primary antibodies
  • Description
    Rabbit polyclonal to MNK1 (phospho T250)
  • Specificityab111434 detects endogenous levels of MNK1 only when phosphorylated at threonine 250 (Human: Thr250; Mouse: Thr209; Rat: Thr197).
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from Human MNK1 around the phosphorylation site of threonine 250 (I-T-TP-P-E).

  • Positive control
    • Human breast carcinoma tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab111434 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMay play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase.
  • Cellular localizationCytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MAP kinase interacting kinase 1 antibody
    • MAP kinase interacting serine/threonine kinase 1 antibody
    • MAP kinase signal integrating kinase 1 antibody
    • MAP kinase signal-integrating kinase 1 antibody
    • MAP kinase-interacting serine/threonine-protein kinase 1 antibody
    • MAPK signal integrating kinase 1 antibody
    • MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1 antibody
    • mknk1 antibody
    • MKNK1_HUMAN antibody
    • MNK 1 antibody
    • Mnk1 antibody
    see all

Anti-MNK1 (phospho T250) antibody images

  • ab111434, at 1/50 dilution, staining MNK1 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.

References for Anti-MNK1 (phospho T250) antibody (ab111434)

ab111434 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"