Overview

  • Product nameAnti-MNK1 (phospho T385) antibody [EPR2370]
    See all MNK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2370] to MNK1 (phospho T385)
  • Specificityab109102 recognises MNK1 only when phosphorilated on Threonine 385.
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho synthetic peptide corresponding to residues in the region of T385 of Human MNK1.

  • Positive control
    • HeLa cell lysates, treated with Calyculin A; Human colon tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 51 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionMay play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
    • Tissue specificityUbiquitous.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase.
    • Cellular localizationCytoplasm and Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • MAP kinase interacting kinase 1 antibody
      • MAP kinase interacting serine/threonine kinase 1 antibody
      • MAP kinase signal integrating kinase 1 antibody
      • MAP kinase signal-integrating kinase 1 antibody
      • MAP kinase-interacting serine/threonine-protein kinase 1 antibody
      • MAPK signal integrating kinase 1 antibody
      • MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1 antibody
      • mknk1 antibody
      • MKNK1_HUMAN antibody
      • MNK 1 antibody
      • Mnk1 antibody
      see all

    Anti-MNK1 (phospho T385) antibody [EPR2370] images

    • All lanes : Anti-MNK1 (phospho T385) antibody [EPR2370] (ab109102) at 1/1000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with Calyculin A.

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 51 kDa
    • Immunohistochemical analysis of MNK1 (phospho T385) expression in paraffin-embedded Human colon tissue using ab109102 at 1/250 dilution.

    References for Anti-MNK1 (phospho T385) antibody [EPR2370] (ab109102)

    This product has been referenced in:
    • Attar-Schneider O  et al. Migration and epithelial-to-mesenchymal transition of lung cancer can be targeted via translation initiation factors eIF4E and eIF4GI. Lab Invest 96:1004-15 (2016). Read more (PubMed: 27501049) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab109102.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"