1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/250.
Application notesIs unsuitable for Flow Cyt or IP.
FunctionMay play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Post-translational modificationsDual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase.
Cellular localizationCytoplasm and Cytoplasm. Nucleus.