Recombinant
RabMAb

Anti-MNK1 (phospho T385) antibody [EPR2370] (ab109102)

Overview

  • Product name
    Anti-MNK1 (phospho T385) antibody [EPR2370]
    See all MNK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2370] to MNK1 (phospho T385)
  • Specificity
    ab109102 recognises MNK1 only when phosphorilated on Threonine 385.
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho synthetic peptide corresponding to residues in the region of T385 of Human MNK1.

  • Positive control
    • HeLa cell lysates, treated with Calyculin A; Human colon tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 51 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      May play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase.
    • Cellular localization
      Cytoplasm and Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • MAP kinase interacting kinase 1 antibody
      • MAP kinase interacting serine/threonine kinase 1 antibody
      • MAP kinase signal integrating kinase 1 antibody
      • MAP kinase signal-integrating kinase 1 antibody
      • MAP kinase-interacting serine/threonine-protein kinase 1 antibody
      • MAPK signal integrating kinase 1 antibody
      • MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1 antibody
      • mknk1 antibody
      • MKNK1_HUMAN antibody
      • MNK 1 antibody
      • Mnk1 antibody
      see all

    Images

    • All lanes : Anti-MNK1 (phospho T385) antibody [EPR2370] (ab109102) at 1/1000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with Calyculin A.

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 51 kDa
    • Immunohistochemical analysis of MNK1 (phospho T385) expression in paraffin-embedded Human colon tissue using ab109102 at 1/250 dilution.

    References

    This product has been referenced in:
    • Attar-Schneider O  et al. Migration and epithelial-to-mesenchymal transition of lung cancer can be targeted via translation initiation factors eIF4E and eIF4GI. Lab Invest 96:1004-15 (2016). Read more (PubMed: 27501049) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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