The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
FunctionActivator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L.
Tissue specificityAdrenal gland, bone marrow, brain, lung, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, uterus, colon with mucosa, fetal brain and fetal liver.
Sequence similaritiesBelongs to the MOB1/phocein family.
MOB1 Mps one binder kinase activator like 1A antibody
MOBKL 1A antibody
Mps one binder kinase activator like 1A antibody
Mps one binder kinase activator-like 1A antibody
Protein Mob4A antibody
Anti-MOB4A antibody images
Western blot - MOB4A antibody (ab72757)
All lanes : Anti-MOB4A antibody (ab72757) at 1/500 dilution
Lane 1 : 293 cell extract treated with IFN (2500U/ml,
30mins) Lane 2 : Cos7 cell extract treated with IFN (2500U/ml,
30mins) Lane 3 : HUVEC extract treated with IFN (2500U/ml,
30mins) Lane 4 : 293 cell extract treated with IFN (2500U/ml,
30mins) with immunising peptide at 10 µg
Lysates/proteins at 10 µg per lane.
Predicted band size : 25 kDa Observed band size : 25 kDa Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.
References for Anti-MOB4A antibody (ab72757)
has not yet been referenced specifically in any publications.
Publishing research using ab72757? Please let us know so that we can cite the reference in this datasheet.
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