Anti-MOBK1B antibody - N-terminal (ab171300)

Overview

  • Product name
    Anti-MOBK1B antibody - N-terminal
    See all MOBK1B primary antibodies
  • Description
    Rabbit polyclonal to MOBK1B - N-terminal
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MOBK1B aa 4-33 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9H8S9

  • Positive control
    • 293 cell lysate; 293 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    ab171300 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 25 kDa.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L.
  • Tissue specificity
    Adrenal gland, bone marrow, brain, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, heart, spinal cord, fetal brain and fetal liver.
  • Sequence similarities
    Belongs to the MOB1/phocein family.
  • Information by UniProt
  • Database links
  • Alternative names
    • B1, Mps One Binder kinase activator like 1B (yeast) antibody
    • C2orf6 antibody
    • Chromosome 2 open reading frame 6 antibody
    • FLJ10788 antibody
    • FLJ11595 antibody
    • MATS1 antibody
    • MOB kinase activator 1A antibody
    • MOB1 antibody
    • Mob1 alpha antibody
    • Mob1 homolog 1B antibody
    • MOB1 Mps One Binder homolog A antibody
    • MOB1 Mps One Binder kinase activator like 1B antibody
    • Mob1A antibody
    • MOB4B antibody
    • Mob4B protein antibody
    • MOBK1B antibody
    • Mobkl1b antibody
    • MOL1B_HUMAN antibody
    • Mps one binder kinase activator like 1B antibody
    • Mps one binder kinase activator-like 1B antibody
    • Protein Mob4B antibody
    see all

Images

  • All lanes : Anti-MOBK1B antibody - N-terminal (ab171300) at 1/50 dilution

    Lane 1 : Non-transfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently-transfected with the MOBKL1B gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 25 kDa
  • Flow cytometric analysis of 293 cells (right histogram) labeling MOBK1B using ab171300 at 1/10 dilution, compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab171300 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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