For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit polyclonal to MOBKL2B
Detects endogenous levels of total MOBKL2B protein.
Predicted to work with:
Synthesized peptide derived from an internal sequence of human MOBKL2B.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
May regulate the activity of kinases.
Belongs to the MOB1/phocein family.
Information by UniProt
Mob1 homolog 2b antibody
MOB1 Mps One Binder kinase activator like 2B (yeast) antibody
MOB1 Mps One Binder kinase activator like 2B antibody
Western blot - MOBKL2B antibody (ab72759)
All lanes : Anti-MOBKL2B antibody (ab72759) at 1/500 dilution Lane 1 : Extracts from COLO cells Lane 2 : Extracts from COLO cells with immunising peptide at 5 µg Lysates/proteins at 5 µg per lane. Predicted band size : 25 kDa Observed band size : 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-MOBKL2B antibody(ab72759)
IHC image of ab72759 staining in human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond
TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab72759, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"