Overview

  • Product nameAnti-MOBKL2C antibody
    See all MOBKL2C primary antibodies
  • Description
    Rabbit polyclonal to MOBKL2C
  • SpecificityDetects endogenous levels of total MOBKL2C protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from an internal region of human MOL2C.

  • Positive control
    • RAW264.7 cell extract K562 cell extract

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa).
ELISA 1/40000.

Target

  • RelevanceMOBKL2C is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
  • Database links
  • Alternative names
    • MOB kinase activator 3C antibody
    • Mob1 homolog 2C antibody
    • MOB1E antibody
    • MOB3C antibody
    • Mps One Binder kinase activator like 2C antibody
    see all

Anti-MOBKL2C antibody images

  • All lanes : Anti-MOBKL2C antibody (ab72756) at 1/500 dilution

    Lane 1 : RAW264.7 cell extract
    Lane 2 : K562 cell extract
    Lane 3 : RAW264.7 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 26 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 62 kDa,65 kDa,70 kDa. We are unsure as to the identity of these extra bands.

References for Anti-MOBKL2C antibody (ab72756)

ab72756 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"