Anti-MOCS3 antibody (ab154107)
Key features and details
- Rabbit polyclonal to MOCS3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MOCS3 antibody
See all MOCS3 primary antibodies -
Description
Rabbit polyclonal to MOCS3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Pig -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 220-460 of Human MOCS3 (Uniprot ID: O95396).
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Positive control
- A549, HeLa, HepG2 and HCT116 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 50 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 50 kDa. |
Target
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Function
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. -
Pathway
tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
Cofactor biosynthesis; molybdopterin biosynthesis. -
Sequence similarities
In the N-terminal section; belongs to the hesA/moeB/thiF family. UBA4 subfamily.
Contains 1 rhodanese domain. -
Cellular localization
Cytoplasm > cytosol. - Information by UniProt
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Database links
- Entrez Gene: 27304 Human
- Entrez Gene: 100124378 Pig
- GenBank: BC015939 Human
- Omim: 609277 Human
- SwissProt: O95396 Human
- SwissProt: A5GFZ6 Pig
- Unigene: 159410 Human
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Alternative names
- Adenylyltransferase MOCS3 antibody
- dJ914P20.3 antibody
- MGC9252 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154107 has been referenced in 1 publication.
- Kim KS et al. Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients. Hum Mol Genet 27:837-852 (2018). PubMed: 29309586