Overview

  • Product name
  • Description
    Rabbit polyclonal to MOCS3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 220-460 of Human MOCS3 (Uniprot ID: O95396).

  • Positive control
    • A549, HeLa, HepG2 and HCT116 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab154107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.

Target

  • Function
    Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.
  • Pathway
    tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
    Cofactor biosynthesis; molybdopterin biosynthesis.
  • Sequence similarities
    In the N-terminal section; belongs to the hesA/moeB/thiF family. UBA4 subfamily.
    Contains 1 rhodanese domain.
  • Cellular localization
    Cytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenylyltransferase MOCS3 antibody
    • dJ914P20.3 antibody
    • MGC9252 antibody
    • MOCS 3 antibody
    • MOCS3 antibody
    • MOCS3_HUMAN antibody
    • Molybdenum cofactor synthesis 3 antibody
    • Molybdenum cofactor synthesis protein 3 antibody
    • Molybdopterin synthase sulfurylase antibody
    • Molybdopterin-synthase sulfurtransferase antibody
    • MPT synthase sulfurylase antibody
    • Sulfur carrier protein MOCS2A adenylyltransferase antibody
    • Sulfur carrier protein MOCS2A sulfurtransferase antibody
    • Sulfurtransferase MOCS3 antibody
    • UBA 4 antibody
    • UBA4 ubiquitin activating enzyme E1 homolog antibody
    • UBA4 antibody
    see all

Images

  • Anti-MOCS3 antibody (ab154107) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 50 kDa



    10% SDS PAGE

References

ab154107 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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