Overview

  • Product nameAnti-MOCS3 antibody
    See all MOCS3 primary antibodies
  • Description
    Mouse monoclonal to MOCS3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-461 of Human MOCS3

Properties

Applications

Our Abpromise guarantee covers the use of ab57229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.
    • PathwaytRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
      Cofactor biosynthesis; molybdopterin biosynthesis.
    • Sequence similaritiesIn the N-terminal section; belongs to the hesA/moeB/thiF family. UBA4 subfamily.
      Contains 1 rhodanese domain.
    • Cellular localizationCytoplasm > cytosol.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adenylyltransferase MOCS3 antibody
      • dJ914P20.3 antibody
      • MGC9252 antibody
      • MOCS 3 antibody
      • MOCS3 antibody
      • MOCS3_HUMAN antibody
      • Molybdenum cofactor synthesis 3 antibody
      • Molybdenum cofactor synthesis protein 3 antibody
      • Molybdopterin synthase sulfurylase antibody
      • Molybdopterin-synthase sulfurtransferase antibody
      • MPT synthase sulfurylase antibody
      • Sulfur carrier protein MOCS2A adenylyltransferase antibody
      • Sulfur carrier protein MOCS2A sulfurtransferase antibody
      • Sulfurtransferase MOCS3 antibody
      • UBA 4 antibody
      • UBA4 ubiquitin activating enzyme E1 homolog antibody
      • UBA4 antibody
      see all

    Anti-MOCS3 antibody images



    • Predicted band size : 50 kDa
      MOCS3 antibody (ab57229) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    References for Anti-MOCS3 antibody (ab57229)

    ab57229 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"