Overview

  • Product nameAnti-Moesin antibody [38/87]
    See all Moesin primary antibodies
  • Description
    Mouse monoclonal [38/87] to Moesin
  • Specificityab50007 recognizes the 78 kDa moesin and 80 kDa radixin molecules. It does not react with talin or ezrin.
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-P, Indirect ELISA, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Cow, Human, Pig
  • Immunogen

    Purified bovine uterine moesin

  • Positive control
    • Jurkat whole cells extract.
  • General notes


    The antibody has been used to inhibit both the binding of proteoheparan sulfate to smooth muscle cells, and the infectivity of measles virus.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesThe antibody has been used to inhibit both the binding of proteoheparan sulfate to smooth muscle cells, and the infectivity of measles virus.
  • ClonalityMonoclonal
  • Clone number38/87
  • IsotypeIgG1
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab50007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 68 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Indirect ELISA Use at an assay dependent concentration.
Dot blot Use a concentration of 0.5 - 1 µg/ml.

Target

  • FunctionProbably involved in connections of major cytoskeletal structures to the plasma membrane.
  • Tissue specificityIn all tissues and cultured cells studied.
  • Sequence similaritiesContains 1 FERM domain.
  • Post-translational
    modifications
    Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.
  • Cellular localizationCell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis luminal protein 70 antibody
    • HEL70 antibody
    • Membrane organizing extension spike protein antibody
    • Membrane-organizing extension spike protein antibody
    • MOES_HUMAN antibody
    • Moesin antibody
    • Moesin/anaplastic lymphoma kinase fusion protein antibody
    • Msn antibody
    • MSN/ALK fusion antibody
    see all

Anti-Moesin antibody [38/87] images

  • Immunohistochemistry (PFA-fixed) analysis of adult mouse brain tissue labelling Moesin with ab50007. Moesin is shown to be expressed in the adult RMS (rostral migratory stream). Top - low magnification image from DAB staining shows staining along the RMS. Bottom - confocal analysis of doublelabeling with PSA-NCAM shows expression pattern at the cellular level. Moesin was expressed in a number of PSA-NCAM positive cells of the RMS. 

    Brains were sagitally cut on a sliding microtome at 20μm. Immunostaining was preceded by antigen retrieval in sodium citrate, pH 6.0, for 20 min at 98°C followed by 30 minutes cooling at room temperature. For diaminobenzidine (DAB) staining, sections were washed in tris buffered saline (TBS), incubated for 30 minutes in 0.6% H2O2, blocked for 30 minutes with 3% normal donkey serum in 0.1% Triton X-100, and then incubated in primary antibodies for 48 hours at 4°C.

    For fluorescent immunolabeling, free-floating sections were blocked for 30 minutes in 3% normal donkey serum in 0.1% Triton X-100, and then incubated for 48 hours at 4°C in primary antibody. After rinsing in TBS, sections were incubated for 1 hour with biotinylated donkey anti-mouse IgG. Sections were then washed and incubated with Alexa Fluor-conjugated secondary antibodies. ToPro3 was used as a nuclear counterstain.

References for Anti-Moesin antibody [38/87] (ab50007)

This product has been referenced in:
  • Ruppersburg CC & Hartzell HC The Ca2+-activated Cl- channel ANO1/TMEM16A regulates primary ciliogenesis. Mol Biol Cell 25:1793-807 (2014). ICC/IF . Read more (PubMed: 24694595) »
  • Cooray SN  et al. Ligand-specific conformational change of the G-protein-coupled receptor ALX/FPR2 determines proresolving functional responses. Proc Natl Acad Sci U S A 110:18232-7 (2013). WB ; Human . Read more (PubMed: 24108355) »

See all 8 Publications for this product

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Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Hamster Cell lysate - whole cell (CHO)
Loading amount 30 µg
Specification CHO
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Aug 04 2008

Application Western blot
Sample Monkey Cell lysate - whole cell (hepatocyte)
Loading amount 25 µg
Specification hepatocyte
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Jun 13 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (hepatocyte)
Specification hepatocyte
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Jun 13 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Hepatocyte)
Loading amount 25 µg
Specification Hepatocyte
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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Verified customer

Submitted Jun 13 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepG2)
Loading amount 25 µg
Specification HepG2
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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Submitted Jun 13 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"