Overview

  • Product name
    Anti-Moesin antibody [38/87]
    See all Moesin primary antibodies
  • Description
    Mouse monoclonal [38/87] to Moesin
  • Specificity
    ab50007 recognizes the 78 kDa moesin and 80 kDa radixin molecules. It does not react with talin or ezrin.
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-P, Indirect ELISA, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Cow, Human, Pig
  • Immunogen

    Purified bovine uterine moesin

  • Positive control
    • Jurkat whole cells extract.
  • General notes

    The antibody has been used to inhibit both the binding of proteoheparan sulfate to smooth muscle cells, and the infectivity of measles virus.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab151542 as a replacement.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    The antibody has been used to inhibit both the binding of proteoheparan sulfate to smooth muscle cells, and the infectivity of measles virus.
  • Clonality
    Monoclonal
  • Clone number
    38/87
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 68 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Indirect ELISA Use at an assay dependent concentration.
Dot blot Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function
    Probably involved in connections of major cytoskeletal structures to the plasma membrane.
  • Tissue specificity
    In all tissues and cultured cells studied.
  • Sequence similarities
    Contains 1 FERM domain.
  • Post-translational
    modifications
    Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.
  • Cellular localization
    Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis luminal protein 70 antibody
    • HEL70 antibody
    • Membrane organizing extension spike protein antibody
    • Membrane-organizing extension spike protein antibody
    • MOES_HUMAN antibody
    • Moesin antibody
    • Moesin/anaplastic lymphoma kinase fusion protein antibody
    • Msn antibody
    • MSN/ALK fusion antibody
    see all

Images

  • Immunohistochemistry (PFA-fixed) analysis of adult mouse brain tissue labelling Moesin with ab50007. Moesin is shown to be expressed in the adult RMS (rostral migratory stream). Top - low magnification image from DAB staining shows staining along the RMS. Bottom - confocal analysis of doublelabeling with PSA-NCAM shows expression pattern at the cellular level. Moesin was expressed in a number of PSA-NCAM positive cells of the RMS. 

    Brains were sagitally cut on a sliding microtome at 20μm. Immunostaining was preceded by antigen retrieval in sodium citrate, pH 6.0, for 20 min at 98°C followed by 30 minutes cooling at room temperature. For diaminobenzidine (DAB) staining, sections were washed in tris buffered saline (TBS), incubated for 30 minutes in 0.6% H2O2, blocked for 30 minutes with 3% normal donkey serum in 0.1% Triton X-100, and then incubated in primary antibodies for 48 hours at 4°C.

    For fluorescent immunolabeling, free-floating sections were blocked for 30 minutes in 3% normal donkey serum in 0.1% Triton X-100, and then incubated for 48 hours at 4°C in primary antibody. After rinsing in TBS, sections were incubated for 1 hour with biotinylated donkey anti-mouse IgG. Sections were then washed and incubated with Alexa Fluor-conjugated secondary antibodies. ToPro3 was used as a nuclear counterstain.

References

This product has been referenced in:
  • Ji X  et al. Retinal Pigment Epithelium Atrophy 1 (rpea1): A New Mouse Model With Retinal Detachment Caused by a Disruption of Protein Kinase C, ?. Invest Ophthalmol Vis Sci 57:877-88 (2016). Read more (PubMed: 26978024) »
  • Ruppersburg CC & Hartzell HC The Ca2+-activated Cl- channel ANO1/TMEM16A regulates primary ciliogenesis. Mol Biol Cell 25:1793-807 (2014). ICC/IF . Read more (PubMed: 24694595) »

See all 11 Publications for this product

Customer reviews and Q&As

Vielen Dank für Ihre Anfrage.

Als CXCL12(SDF1)-Antikörper könnte ich Ihnen die Kaninchen-polyklonalen ab9797 oder ab109854 empfehlen, die wir für IHC an Paraffinschnitten garantieren können:
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Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Hamster Cell lysate - whole cell (CHO)
Loading amount
30 µg
Specification
CHO
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 04 2008

Application
Western blot
Sample
Monkey Cell lysate - whole cell (hepatocyte)
Loading amount
25 µg
Specification
hepatocyte
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jun 13 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (hepatocyte)
Specification
hepatocyte
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jun 13 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hepatocyte)
Loading amount
25 µg
Specification
Hepatocyte
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jun 13 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2)
Loading amount
25 µg
Specification
HepG2
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jun 13 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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