The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA:Use at an assay dependent dilution. Antibody detection limit dilution 1:16,000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionProbably involved in connections of major cytoskeletal structures to the plasma membrane.
Tissue specificityIn all tissues and cultured cells studied.
Sequence similaritiesContains 1 FERM domain.
Post-translational modificationsPhosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.
Cellular localizationCell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
Rørvig S et al. Proteome profiling of human neutrophil granule subsets, secretory vesicles, and cell membrane: correlation with transcriptome profiling of neutrophil precursors. J Leukoc Biol94:711-21 (2013).
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