Validated using a knockout cell line
Recombinant
RabMAb

Anti-Moesin antibody [EPR2428(2)] (ab151542)

Overview

  • Product name
    Anti-Moesin antibody [EPR2428(2)]
    See all Moesin primary antibodies
  • Description
    Rabbit monoclonal [EPR2428(2)] to Moesin
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • Raji, Jurkat and HeLa cell lysates; Human breast carcinoma tissue; Raji cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab151542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 68 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Probably involved in connections of major cytoskeletal structures to the plasma membrane.
    • Tissue specificity
      In all tissues and cultured cells studied.
    • Sequence similarities
      Contains 1 FERM domain.
    • Post-translational
      modifications
      Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.
    • Cellular localization
      Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
    • Information by UniProt
    • Database links
    • Alternative names
      • Epididymis luminal protein 70 antibody
      • HEL70 antibody
      • Membrane organizing extension spike protein antibody
      • Membrane-organizing extension spike protein antibody
      • MOES_HUMAN antibody
      • Moesin antibody
      • Moesin/anaplastic lymphoma kinase fusion protein antibody
      • Msn antibody
      • MSN/ALK fusion antibody
      see all

    Images



    • Predicted band size : 68 kDa

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Moesin knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: Raji whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab151542 observed at 75 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab151542 was shown to specifically react with Moesin in wild-type HAP1 cells as signal was lost in Moesin knockout cells. Wild-type and Moesin knockout samples were subjected to SDS-PAGE. Ab151542 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Moesin antibody [EPR2428(2)] (ab151542) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 68 kDa
    • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Moesin with ab151542 at 1/100 dilution.
    • Immunofluorescent analysis of Raji cells labeling Moesin with ab151542 at 1/100 dilution.

    References

    ab151542 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (NIH 3T3 cells)
    Loading amount
    30 µg
    Specification
    NIH 3T3 cells
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 02 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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