Probably involved in connections of major cytoskeletal structures to the plasma membrane.
In all tissues and cultured cells studied.
Contains 1 FERM domain.
Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.
Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
Membrane organizing extension spike protein antibody
Membrane-organizing extension spike protein antibody
Moesin/anaplastic lymphoma kinase fusion protein antibody
MSN/ALK fusion antibody
Western blot - Anti-Moesin antibody [EPR2428(2)] (ab151542)
Predicted band size : 68 kDa
Lane 1: Wild-type HAP1 whole cell lysate (20 µg) Lane 2: Moesin knockout HAP1 whole cell lysate (20 µg) Lane 3: HeLa whole cell lysate (20 µg) Lane 4: Raji whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab151542 observed at 75 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab151542 was shown to specifically react with Moesin in wild-type HAP1 cells as signal was lost in Moesin knockout cells. Wild-type and Moesin knockout samples were subjected to SDS-PAGE. Ab151542 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Test Target DM antibody [EPR2428(2)] (ab151542)
All lanes : Anti-Moesin antibody [EPR2428(2)] (ab151542) at 1/1000 dilution
Lane 1 : Raji cell lysate Lane 2 : Jurkat cell lysate Lane 3 : HeLa cell lysate