Recombinant
RabMAb

Anti-Moesin antibody [EPR2429(2)] (ab169789)

Overview

  • Product name
    Anti-Moesin antibody [EPR2429(2)]
    See all Moesin primary antibodies
  • Description
    Rabbit monoclonal [EPR2429(2)] to Moesin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Moesin (Uniprot ID: P26038)

  • Positive control
    • HeLa, Raji, 293T and Jurkat cell lysates; Human lung and stomach tissues; Raji cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR2429(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 68 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Probably involved in connections of major cytoskeletal structures to the plasma membrane.
  • Tissue specificity
    In all tissues and cultured cells studied.
  • Sequence similarities
    Contains 1 FERM domain.
  • Post-translational
    modifications
    Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.
  • Cellular localization
    Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis luminal protein 70 antibody
    • HEL70 antibody
    • Membrane organizing extension spike protein antibody
    • Membrane-organizing extension spike protein antibody
    • MOES_HUMAN antibody
    • Moesin antibody
    • Moesin/anaplastic lymphoma kinase fusion protein antibody
    • Msn antibody
    • MSN/ALK fusion antibody
    see all

Images

  • All lanes : Anti-Moesin antibody [EPR2429(2)] (ab169789) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : standard HRP labeled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 68 kDa

  • Immunoprecipitation analysis of pellet from HeLa cell lysate, using anti-Moesin ab169789 at a 1/10 dilution.
  • Flow cytometric analysis of permeabilized Raji cells labeling ab169789 (red) at a 1/10 dilution, and negative control cells probed with a rabbit IgG (green).
  • Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human lung tissue, labeling Moesin using ab169789 at a 1/100 dilution.
  • Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human stomach tissue, labeling Moesin using ab169789 at a 1/100 dilution.

References

ab169789 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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