The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 68 kDa.
Use a concentration of 0.5 - 1 µg/ml.
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 0.5 - 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use at 0.25-0.5 µg/mg of lysate.
Probably involved in connections of major cytoskeletal structures to the plasma membrane.
In all tissues and cultured cells studied.
Contains 1 FERM domain.
Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.
Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.