Overview

  • Product name
    Anti-Moesin (phospho T558) antibody
    See all Moesin primary antibodies
  • Description
    Rabbit polyclonal to Moesin (phospho T558)
  • Specificity
    ab61109 detects endogenous levels of Moesin only when phosphorylated at threonine 558. Cross-reacts with Ezrin (T567) and Radixin (T564).
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human Moesin around the phosphorylation site of threonine 558 (Y-K-TP-L-R).

  • Positive control
    • Extracts NIH/3T3 Jurkat cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).

Target

  • Function
    Probably involved in connections of major cytoskeletal structures to the plasma membrane.
  • Tissue specificity
    In all tissues and cultured cells studied.
  • Sequence similarities
    Contains 1 FERM domain.
  • Post-translational
    modifications
    Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.
  • Cellular localization
    Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis luminal protein 70 antibody
    • HEL70 antibody
    • Membrane organizing extension spike protein antibody
    • Membrane-organizing extension spike protein antibody
    • MOES_HUMAN antibody
    • Moesin antibody
    • Moesin/anaplastic lymphoma kinase fusion protein antibody
    • Msn antibody
    • MSN/ALK fusion antibody
    see all

Images

  • All lanes : Anti-Moesin (phospho T558) antibody (ab61109) at 1/500 dilution

    Lane 1 : NIH/3T3 Jurkat Cells
    Lane 2 : NIH/3T3 Jurkat Cells with immunising Human Moesin Phosphopeptide


    Predicted band size : 68 kDa
    Observed band size : 68 kDa

References

This product has been referenced in:
  • Cooray SN  et al. Ligand-specific conformational change of the G-protein-coupled receptor ALX/FPR2 determines proresolving functional responses. Proc Natl Acad Sci U S A 110:18232-7 (2013). WB ; Human . Read more (PubMed: 24108355) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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