Overview

  • Product name
  • Description
    Rabbit polyclonal to MOGAT2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide within residues: LLGIIVGGAQ EALDARPGSF TLLLRNRKGF VRLALTHGAP LVPIFSFGEN, corresponding to internal sequence amino acids 180-229 of Human MOGAT2 (NP_079374)

  • Positive control
    • Human fetal liver lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab84656 is purified by a peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Has a preference toward monoacylglycerols containing unsaturated fatty acids in an order of C18:3 > C18:2 > C18:1 > C18:0. Plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. May play a role in diet-induced obesity.
  • Tissue specificity
    Highly expressed in liver, small intestine, colon, stomach and kidney.
  • Pathway
    Glycerolipid metabolism; triacylglycerol biosynthesis.
  • Sequence similarities
    Belongs to the diacylglycerol acyltransferase family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2 acylglycerol O acyltransferase 2 antibody
    • 2-acylglycerol O-acyltransferase 2 antibody
    • Acyl CoA:monoacylglycerol acyltransferase 2 antibody
    • Acyl-CoA:monoacylglycerol acyltransferase 2 antibody
    • DC5 antibody
    • DGAT2L5 antibody
    • Diacylglycerol acyltransferase 2 like protein 5 antibody
    • Diacylglycerol acyltransferase 2-like protein 5 antibody
    • Diacylglycerol O acyltransferase candidate 5 antibody
    • Diacylglycerol O-acyltransferase candidate 5 antibody
    • EC 2.3.1.22 antibody
    • FLJ22644 antibody
    • hDC5 antibody
    • hMGAT2 antibody
    • Mgat1l antibody
    • MGAT2 antibody
    • MGC119183 antibody
    • MGC119184 antibody
    • MGC119185 antibody
    • MGC189143 antibody
    • mogat2 antibody
    • MOGT2_HUMAN antibody
    • Monoacylglycerol O acyltransferase 1 like antibody
    • Monoacylglycerol O acyltransferase 2 antibody
    • Monoacylglycerol O-acyltransferase 2 antibody
    see all

Images

  • Anti-MOGAT2 antibody (ab84656) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 38 kDa

References

ab84656 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Jejunum)
Gel Running Conditions
Reduced Denaturing
Loading amount
60 µg
Specification
Jejunum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Jul 14 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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