Overview

  • Product name
    Anti-MOGT1 antibody
  • Description
    Rabbit polyclonal to MOGT1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide designed within C terminal amino acids 288-337 (PIPVRQTLNP TQEQIEELHQ TYMEELRKLF EEHKGKYGIP EHETLVLK) of Human MOGT1 (NP_477513)

  • Positive control
    • Fetal liver lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

Target

  • Function
    Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Probably not involved in absorption of dietary fat in the small intestine.
  • Pathway
    Glycerolipid metabolism; triacylglycerol biosynthesis.
  • Sequence similarities
    Belongs to the diacylglycerol acyltransferase family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 0610030A14Rik antibody
    • 1110064N14Rik antibody
    • 2-acylglycerol O-acyltransferase 1 antibody
    • Acyl CoA:monoacylglycerol acyltransferase 1 antibody
    • Acyl-CoA:monoacylglycerol acyltransferase 1 antibody
    • DGAT2L antibody
    • DGAT2L1 antibody
    • Diacylglycerol acyltransferase 2 like antibody
    • Diacylglycerol acyltransferase 2-like protein 1 antibody
    • Diacylglycerol O acyltransferase 2 like 1 antibody
    • Diacylglycerol O-acyltransferase candidate 2 antibody
    • hDC2 antibody
    • mDC2 antibody
    • MGAT1 antibody
    • MGC118035 antibody
    • MOGAT1 antibody
    • MOGT1_HUMAN antibody
    • Monoacylglycerol O acyltransferase 1 antibody
    • Monoacylglycerol O-acyltransferase 1 antibody
    • OTTMUSP00000025181 antibody
    see all

Images

  • Anti-MOGT1 antibody (ab81177) at 1 µg/ml + fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References

ab81177 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (stomach)
Gel Running Conditions
Reduced Denaturing
Loading amount
60 µg
Treatment
RIPA buffer is used for lysis buffer
Specification
stomach
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 08 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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