Anti-Mohawk homeobox antibody (ab66939)

Overview

  • Product name
    Anti-Mohawk homeobox antibody
    See all Mohawk homeobox primary antibodies
  • Description
    Rabbit polyclonal to Mohawk homeobox
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: EGYSTPAHHT VIKGESSAIK AGGRPESRAA EDYVSPPKYK SSLLNRYLND, corresponding to internal sequence amino acids 181-230 of Mouse Mohawk homeobox

  • Positive control
    • NIH 3T3 lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab66939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    May act as a morphogenetic regulator of cell adhesion.
  • Sequence similarities
    Belongs to the TALE/IRO homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9430023B20Rik antibody
    • C10orf48 antibody
    • Homeobox protein Mohawk antibody
    • IFRX antibody
    • Iroquois family related homeodomain protein antibody
    • iroquois homeobox protein like 1 antibody
    • IRXL1 antibody
    • MGC39616 antibody
    • MKX antibody
    • MKX_HUMAN antibody
    • mohawk homeobox antibody
    • OTTHUMP00000019374 antibody
    see all

Images

  • Anti-Mohawk homeobox antibody (ab66939) at 2.5 µg/ml + NIH 3T3 lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    Additional bands at : ~75 kDa. We are unsure as to the identity of these extra bands.

References

ab66939 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (Olfactory Stem Cells of Rabbit)
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory Stem Cells of Rabbit
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
Username

Antoine Veron

Verified customer

Submitted Jul 22 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Olfactory Stem Cells (GFP))
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory Stem Cells (GFP)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
Username

Antoine Veron

Verified customer

Submitted May 31 2016

Application
Western blot
Sample
Horse Cell lysate - whole cell (various cell type)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
various cell type
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 23 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (Olfactory stem cells (tenocytes differentiation))
Permeabilization
Yes - 0,1 % Triton X100
Specification
Olfactory stem cells (tenocytes differentiation)
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Antoine Veron

Verified customer

Submitted Aug 12 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (Olfactory stem cells (differentiation tendon))
Permeabilization
Yes - 0,1% Triton 100X
Specification
Olfactory stem cells (differentiation tendon)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Antoine Veron

Verified customer

Submitted Jun 19 2015

Thank you for contacting us and your interest in our products.

As you have seen from our website, the anti-OAF antibody (ab116332) has not as yet been used in detecting themouse protein as you are hoping to do. This does not mean that it woul...

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