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|Sample type||Average %||Range|
|Serum||>=85||12.5ng/ml - 400ng/ml|
Abcam’s Albumin Monkey ELISA kit (ab190533) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Albumin in monkey serum, plasma and urine.
In this assay the Albumin present in samples reacts with the anti-Albumin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Albumin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Albumin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Albumin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Albumin in the test sample. The quantity of Albumin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Albumin (Alb) is a polyfunctional protein contributing to homeostasis through mechanisms of hemodynamics, transport and nutrition. Albumin is found both intra and extravascularly in all mammals and many lower vertebrates. It is a molecule of about 67,000 daltons, synthesized by the liver. Normally only very trace amounts of albumin escape reabsorption by kidney glomeruli and is excreted into the urine. Many occult diseases can cause kidney damage which may result in excessive amounts of serum proteins, including albumin, to be excreted by the kidney and into the urine. This ELISA kit can be used to measure albumin in serum, tissue extracts and other biological fluids.
The calibrator used in this kit is of human origin. It is quantitated with purified Cynomolgus monkey albumin and behaves identically with it.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 50ml|
|5X Diluent Concentrate||1 x 50ml|
|Albumin Monkey Antibody coated microwells||1 unit|
|Lyophilized Albumin Monkey Calibrator||1 vial|
|Albumin Monkey HRP Conjugate||1 vial|
|Stop Solution||1 x 12ml|
|Chromogen Substrate Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab190533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab190533 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"