Overview

  • Product name
    Monkey E-selectin ELISA Kit
    See all E-selectin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4 < 5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 4 < 10%
  • Sample type
    Cell culture supernatant, Serum
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.4 U/ml
  • Range
    1.9 U/ml - 60 U/ml
  • Recovery

    85 %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Monkey
  • Product overview

    Abcam’s E-selectin monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of monkey E-selectin concentrations in cell culture supernatant and serum.

    E-selectin specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with an HRP-conjugated E-selectin detection antibody. The microplate is then incubated at room temperature. After the removal of unbound proteins by washing TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of E-selectin captured on the plate.

  • Notes

    Standard is recombinant protein produced in E.coli.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab119523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab119523.

Protocols

References

ab119523 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

The product has 120 days guarantee period in Thailand. The 120 days starts form the date of purchase. Expiry date for biological compounds is difficult to guess so we provide guarantee rather than expiry date.
Please store the components as advise...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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