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Abcam’s E-selectin monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of monkey E-selectin concentrations in cell culture supernatant and serum.
E-selectin specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with an HRP-conjugated E-selectin detection antibody. The microplate is then incubated at room temperature. After the removal of unbound proteins by washing TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of E-selectin captured on the plate.
Standard is recombinant protein produced in E.coli.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||2 units|
|E-selectin Standard lyophilized (120 U/mL upon reconstitution)||2 vials|
|HRP Conjugated anti-E-selectin monoclonal antibody||1 x 150µl|
|Microplate coated with monoclonal antibody to E-selectin (12 x 8 wells)||1 unit|
|Sample Diluent||1 x 12ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119523 has not yet been referenced specifically in any publications.